PMID: 9188188Apr 1, 1997Paper

Evidence for peptide synthesis in the course of in vitro proteolysis

Die Nahrung
P LorenzenH Brückner

Abstract

The present paper reports on studies concerned with furnishing evidence for peptide synthesis in the course of in vitro proteolysis. To this end, the oxidized chain B from insulin (INS) (S = 5% in demineralized water) was subjected to tryptic proteolysis (E/S = 1/50; pH 5; 37 degrees C; 24 h). HPLC-as well as amino acid-and sequence analytical studies have shown that the heptapeptide INS 23-29 (Gly-Phe-Phe-Tyr-Thr-Pro-Lys) liberated by way of hydrolysis is linked by tryptic synthesis via transpeptidation or condensation to form a dimer which accounts for 15% of the amount of monomer. The results of the model trials show clearly that during in vitro proteolysis chemical reactions beyond hydrolytic cleavage of peptide bonds take place. In principle, plastein-like reactions (transpeptidation, condensation) can occur during each in vitro proteolysis.

References

Dec 1, 1988·Biological Chemistry Hoppe-Seyler·H Meisel, H Frister
Feb 9, 1987·FEBS Letters·C CarlesB Ribadeau-Dumas
Jul 17, 1974·Biochimica Et Biophysica Acta·M YamashitaM Fujimaki
Dec 1, 1965·Justus Liebigs Annalen der Chemie·H Determann, R Köhler
Feb 1, 1996·Die Nahrung·P Chr LorenzenE Schlimme
Aug 19, 1950·Nature·C S HANES, F J R HIRD

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.