PMID: 9190214Feb 1, 1997Paper

Evidence for physical and functional interactions among two Saccharomyces cerevisiae SH3 domain proteins, an adenylyl cyclase-associated protein and the actin cytoskeleton

Molecular Biology of the Cell
T Lila, David G Drubin

Abstract

In a variety of organisms, a number of proteins associated with the cortical actin cytoskeleton contain SH3 domains, suggesting that these domains may provide the physical basis for functional interactions among structural and regulatory proteins in the actin cytoskeleton. We present evidence that SH3 domains mediate at least two independent functions of the Saccharomyces cerevisiae actin-binding protein Abp1p in vivo. Abp1p contains a single SH3 domain that has recently been shown to bind in vitro to the adenylyl cyclase-associated protein Srv2p. Immunofluorescence analysis of Srv2p subcellular localization in strains carrying mutations in either ABP1 or SRV2 reveals that the Abp1p SH3 domain mediates the normal association of Srv2p with the cortical actin cytoskeleton. We also show that a site in Abp1p itself is specifically bound by the SH3 domain of the actin-associated protein Rvs167p. Genetic analysis provides evidence that Abp1p and Rvs167p have functions that are closely interrelated. Abp1 null mutations, like rvs167 mutations, result in defects in sporulation and reduced viability under certain suboptimal growth conditions. In addition, mutations in ABP1 and RVS167 yield similar profiles of genetic "synthetic lethal" i...Continue Reading

References

May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J E GerstM Wigler
Oct 1, 1991·Current Opinion in Genetics & Development·J R Broach
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1985·Annual Review of Cell Biology·T P StosselH L Yin
Dec 1, 1988·The Journal of Cell Biology·D G DrubinD Botstein
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Jan 1, 1986·Annual Review of Biochemistry·T D Pollard, J A Cooper
Aug 1, 1994·Cellular Signalling·K A Mintzer, J Field
Apr 1, 1995·Current Biology : CB·B J Mayer, M J Eck
Aug 1, 1995·The Journal of Cell Biology·A E GammieM D Rose
Jan 1, 1995·Nature Structural Biology·D C AmbergD Botstein
May 1, 1995·The Journal of Cell Biology·D J Lew, S I Reed
Feb 1, 1995·Current Opinion in Cell Biology·H Q SunH L Yin
Feb 1, 1995·Current Opinion in Cell Biology·W Sullivan, W E Theurkauf
Feb 1, 1994·Current Opinion in Cell Biology·M D WelchD G Drubin
Jan 1, 1993·Cell Motility and the Cytoskeleton·E L de HostosG Gerisch
Mar 1, 1993·The Journal of Cell Biology·D J Lew, S I Reed
Dec 18, 1995·Experientia·A A Noegel, J E Luna
Jan 25, 1996·Nature·P S McPhersonP De Camilli
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·C DavidP de Camilli
Nov 1, 1995·Molecular Biology of the Cell·A Moon, D G Drubin
Feb 9, 1996·Cell·D G Drubin, W J Nelson
Feb 9, 1996·Cell·D A Lauffenburger, A F Horwitz

❮ Previous
Next ❯

Citations

May 10, 2013·Journal of Biomolecular NMR·Alexandra AhlnerPatrik Lundström
Aug 28, 1998·Current Opinion in Cell Biology·B WendlandH Riezman
Dec 4, 2001·Current Opinion in Microbiology·B L Goode, A A Rodal
Mar 6, 1999·Current Opinion in Microbiology·S J Palmieri, B K Haarer
May 14, 2011·Cell Death and Differentiation·A Smertenko, V E Franklin-Tong
Dec 13, 2005·Nature Reviews. Molecular Cell Biology·Michael J Deeks, Patrick J Hussey
Mar 14, 1998·Molecular Biology of the Cell·K D Novak, M A Titus
Aug 7, 1998·Molecular Biology of the Cell·P LappalainenD G Drubin
Jul 15, 2003·Molecular Biology of the Cell·Helena FriesenBrenda Andrews
Nov 5, 2003·Molecular Biology of the Cell·Angelika A NoegelCornelis J Weijer
Mar 9, 2004·Molecular Biology of the Cell·Enni BertlingPekka Lappalainen
Jan 6, 2006·Molecular Biology of the Cell·Helena FriesenBrenda Andrews
Jun 1, 2007·Molecular Biology of the Cell·Faisal ChaudhryChristopher J Staiger
Feb 24, 2001·Protein Science : a Publication of the Protein Society·A Rath, A R Davidson
Feb 7, 2001·Molecular and Cellular Biology·C M AslesonJ Berman
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·James B Moseley, Bruce L Goode
Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·Gang RenAlan L Munn
Sep 4, 2008·Journal of Bioinformatics and Computational Biology·Natalia SinitsinaOlga Sokolova
Aug 22, 2008·Journal of Cell Science·Changhui WangJeffrey Field
Dec 16, 2004·Environmental Health Perspectives·Chad M VezinaJames R Olson
Jan 23, 2004·PLoS Biology·Christiane LandgrafGianni Cesareni
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·B C OsmondP W Robbins

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.