PMID: 39895Aug 1, 1979

Evidence for pili-mediated adherence of Klebsiella pneumoniae to rat bladder epithelial cells in vitro

Infection and Immunity
R C FaderC P Davis


The possible role of pili in the pathogenesis of urinary tract infections caused by Klebsiella pneumoniae was studied in an in vitro mixture of a phosphate-buffered saline suspension of rat bladder epithelial cells and phosphate-buffered saline-washed K. pneumoniae. Nonpiliated and piliated populations derived from a single K. pneumoniae strain were obtained by controlling the total time of growth in broth medium. The piliated phase demonstrated a significant increase in adherence when compared to the nonpiliated phase. Incubation of the bacteria and epithelial cell mixture at 4 and 37 degrees C resulted in no differences in adherence; optimal adherence occurred at pH 5. Pretreatment of the bacteria with enzymes to destroy the pili resulted in a decrease in adherence, as did killing the bacteria by various means before adherence testing. Pretreatment of the epithelial cells with certain saccharides inhibited bacterial adherence. Finally, a 96% decrease in adherence was observed after coincubation of bacteria and epithelial cells with papain-treated antipili antibodies. Thus, it appears that pili on the surface of K. pneumoniae mediate attachment of the bacteria to rat bladder epithelial cells.


Mar 1, 1986·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·T FrymusK Petzoldt
Jan 1, 1980·Infection·B Sugarman
Jan 15, 1982·Mycopathologia·R L Sandin, A L Rogers
Dec 1, 1983·European Journal of Clinical Microbiology·S J Cryz
Aug 24, 2012·Future Microbiology·Caitlin N Murphy, Steven Clegg
Jan 1, 1980·Microbiology and Immunology·K OkamotoA Miyama
Aug 1, 1983·The Journal of Applied Bacteriology·D C Old, R A Adegbola

Related Concepts

Pathogenic Aspects
Enzymes, antithrombotic
Squamous Transitional Epithelial Cell Count
Rat Urinary Bladder
Urinary Tract Infection
Klebsiella pneumoniae
Phosphate Measurement

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