PMID: 8967352Apr 1, 1996Paper

Evidence for presence and hormonal regulation of protein phosphatase inhibitor-1 in ventricular cardiomyocyte

The American Journal of Physiology
R C GuptaH N Sabbah

Abstract

Protein phosphatase inhibitor-1 (PPI-1) has been shown to be present in heart tissue and smooth muscle. Whether PPI-1 is present in cardiomyocytes is not known. The purpose of this study was to determine whether PPI-1 is present and is hormonally regulated in cardiomyocytes. A trichloroacetic acid (TCA) extract enriched in PPI-1 was isolated from guinea pig ventricular cardiomyocytes. The TCA extract inhibited the activity of type 1 protein phosphatase by 20 +/- 4% (n = 3 expts). On phosphorylation by the catalytic subunit of adenosine 3', 5'-cyclic monophosphate-dependent protein kinase, the extent of this inhibition was augmented to 4.5-fold. Dephosphorylation of the phosphorylated TCA extract by type 2 protein phosphatase reduced inhibition to 2 +/- 0.2% (n = 3 expts). To determine whether isoproterenol increases phosphorylation of PPI-1 in cardiomyocytes, the TCA extracts were prepared from cardiomyocytes treated with 1 microM isoproterenol and from untreated cardiomyocytes. The inhibitory activity of the TCA extract in untreated cardiomyocytes was 25 +/- 3% (n = 3 expts) and increased to 75 +/- 2% (n = 3 expts) in isoproterenol-treated cardiomyocytes. With the use of a rabbit skeletal muscle PPI-1 antibody, immunoblots of ...Continue Reading

Citations

May 25, 2002·Molecular and Cellular Biology·Andrew N CarrEvangelia G Kranias
Aug 12, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Nicole BrüchertJoachim Neumann
May 1, 2021·Antioxidants·Friederike CuelloSimon Diering

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