Evidence for presence of insulin receptors in rat islets of Langerhans

The Journal of Clinical Investigation
E J Verspohl, H P Ammon


Binding of insulin to islets of Langerhans was studied. It was found that "specific" binding of [125I]insulin ("specific" binding equals total binding minus nonspecific binding) was saturable with respect to time and insulin concentration and depended on the number of incubated islets. Furthermore, bound insulin was displaced by native insulin in a dose-dependent manner. Bound [125I]insulin was easily dissociated and there was little [125I]insulin degradation both in the incubation medium and during the processes of binding and dissociation. Scatchard analysis of experiments with increasing [125I]insulin concentration and with displacement of insulin binding by native insulin revealed "high affinity" binding sites with a dissociation constant of 0.461 +/- 0.08 n M and 3.5 X 10(6) high affinity binding sites per islet. There also existed "low affinity" binding sites with dissociation constant (Kd) of 43.9 +/- 11.6 nM and 5.9 X 10(7) low affinity binding sites. High affinity binding sites of islets from rats pretreated with alloxan decreased by about one half, whereas Kd was unaffected. Because the Kd of specific high affinity binding and mean effective dose (ED50) of the biological effects of insulin on normal pancreatic islets ...Continue Reading


May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G S PattonR H Unger
Jun 1, 1977·The Journal of Clinical Endocrinology and Metabolism·H H HatfieldR K Kalkhoff
Jul 16, 1971·Biochemical and Biophysical Research Communications·P CuatrecasasF Krug
Sep 1, 1974·Biochemical Pharmacology·P Cuatrecasas
Nov 15, 1972·Biochemical and Biophysical Research Communications·J M HammondW H Daughaday
Aug 17, 1973·Biochimica Et Biophysica Acta·S Gammeltoft, J Gliemann
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·P Cuatrecasas


Mar 1, 1986·Naunyn-Schmiedeberg's Archives of Pharmacology·E J VerspohlI D Goldfine
Jan 1, 1994·Peptides·E J VerspohlJ J Knittel
May 27, 1982·Biochimica Et Biophysica Acta·E J VerspohlH P Ammon
Aug 1, 1983·The Journal of Cell Biology·D R KaplanM L McDaniel
May 17, 2008·Annual Review of Nutrition·Ingo B LeibigerPer-Olof Berggren
Aug 1, 1989·The Journal of Clinical Investigation·M AmherdtL Orci
Apr 1, 2010·Journal of Nutritional Science and Vitaminology·Hanae YamazakiWalter S Zawalich
Oct 15, 1984·Experientia·D Pipeleers
Nov 26, 2002·Journal of Cellular Physiology·E P HaberA R Carpinelli
Mar 12, 2008·Life Sciences·Flavio FranciniMaría I Borelli
Dec 23, 2014·Basic & Clinical Pharmacology & Toxicology·Anne Wuttke
May 1, 1986·Diabetes Research and Clinical Practice·E J VerspohlH P Ammon
Oct 30, 1984·Biochemical and Biophysical Research Communications·P G Colman, L C Harrison
Dec 10, 1999·Cell Biochemistry and Function·H KlossE J Verspohl
Dec 3, 2002·FEBS Letters·Ingo B LeibigerPer-Olof Berggren
Feb 26, 1999·The Journal of Biological Chemistry·C A AspinwallR T Kennedy
May 16, 2002·The Journal of Biological Chemistry·Walter S Zawalich, Kathleen C Zawalich
Jul 1, 1985·Cell Biochemistry and Function·H P Ammon, M Mark
May 1, 1996·Pharmazie in unserer Zeit·H P Ammon
Mar 11, 2020·International Journal of Molecular Sciences·Nadia Rachdaoui
Sep 18, 2003·American Journal of Physiology. Endocrinology and Metabolism·María I BorelliJuan José Gagliardino
Jun 8, 2017·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Pijush Kumar PaulRoongnapa Suedee

Related Concepts

Metabolic Biotransformation
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