Evidence for programmed odontoblast process retraction after dentine exposure in the rat incisor

Archives of Oral Biology
A MahdeeJ I Gillespie

Abstract

To re-examine the morphology and potential functions of odontoblasts in intact rat incisors and after cavity preparation into dentine. Intact incisors were fixed, decalcified, snap frozen and sectioned (10μm), before staining with rhodamine phalloidin or antibodies for cyto-skeletal proteins: vimentin and actin, ion transporter: NaK-ATPase, and dendritic cell marker: OX6. Samples with cavity were processed similarly and stained for actin and vimentin before comparing the lengths of odontoblast processes (OP) at baseline, 3h and 24h (n=5 for each group). Actin was expressed through the full length of OP, while vimentin immunoreactivity was not uniform, with 4 distinct regions. OP showed morphological complexity with fine branches emanating within different regions of dentine. Novel actin-positive tree-like OP were identified within predentine which reduced in intensity and length toward the incisal portion of the tooth. Specimens with cavities showed time-dependant pulpal retraction of OP. Differences in structural antibody expression suggest functional variations in OP within different regions of dentine. The role of actin positive OP in predentine is not known, but could be related to dentine deposition, cellular stability or ...Continue Reading

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Citations

Nov 16, 2020·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Ninna ShuhaibarMark Terasaki

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