PMID: 8944762Nov 1, 1996Paper

Evidence for propeptide-assisted folding of the calcium-dependent protease of the cyanobacterium Anabaena

European Journal of Biochemistry
K BaierW Lockau

Abstract

The Ca(2+)-dependent protease of the cyanobacterium Anabaena variabilis is a cytoplasmic enzyme with a substrate specificity like trypsin. Its previously published DNA sequence [Maldener, I., Lockau, W., Cai, Y. & Wolk, C. P. (1991) Mol. Gen. Genet. 225, 113-120] contained a sequencing error. Here we report the corrected sequence which shows, that the Ca(2+)-protease belongs to the family of subtilases (subtilisin-like serine proteases). Consistent with its cytoplasmic localization, a pre-sequence is not found. The enzyme is produced as a precursor with a large amino-terminal propeptide. Expression of the pro-region and mature region (protease domain) in Escherichia coli cells in trans demonstrates that formation of the active enzyme requires the propeptide. The results demonstrate that propeptide-assisted protein folding also occurs with cytoplasmic enzymes, in support of the hypothesis that this mechanism is a widespread phenomenon.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1977·Annual Review of Biochemistry·J Kraut
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J R Winther, P Sørensen
May 1, 1995·Molecular Microbiology·J Eder, A R Fersht
Jan 1, 1994·Methods in Enzymology·N D Rawlings, A J Barrett
Nov 1, 1993·Trends in Biochemical Sciences·U Shinde, M Inouye
Jan 1, 1994·Trends in Biochemical Sciences·F U HartlT Langer
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·U ShindeM Inouye
Nov 16, 1990·Science·R J Ellis

❮ Previous
Next ❯

Citations

Sep 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Shun-ichi TanakaShigenori Kanaya
Oct 1, 2010·Biomolecular Concepts·Ilya V DemidyukSergey V Kostrov
Jul 9, 2002·The Journal of Biological Chemistry·Hannah M Linden, Kenneth Kaushansky
Mar 9, 2018·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Neelam DubeySuvendra Nath Bagchi
Dec 12, 2002·Chemical Reviews·Philip N Bryan
Jan 17, 2007·Biochemistry·Kathryn E FisherPhilip N Bryan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.