Evidence for protein dielectric relaxations in reaction centers associated with the primary charge separation detected from Rhodospirillum rubrum chromatophores by combined photovoltage and absorption measurements in the 1-15 ns time range

Biochemistry
H W TrisslM Lapin

Abstract

Fast photovoltage measurements in Rhodospirillum rubrum chromatophores in the nanosecond time range, escorted by time-resolved absorption measurements, are described. Under reducing conditions, the photovoltage decayed significantly faster than the spectroscopically detected charge recombination of the radical pair P(+)H(A)(-). This indicates the occurrence of considerable dielectric relaxations. Our data and data from the literature were analyzed by means of a reaction scheme consisting of three states, namely, A, P, and P(+)H(A)(-). A time-dependent DeltaG(t) was introduced by assuming a time-dependent rate constant of the back-reaction, k(-1)(t). With the exception of the latter rate constant, all other parameters of the model are reliably known within narrow limits. This allowed us to distinguish between the three cases assumed for DeltaG degrees (t): (1)DeltaG degrees (t) = constant; (2)DeltaG degrees (t) as published by Peloquin et al. [Peloquin, J. M., Williams, J. C., Lin, X. M., Alden, R. G., Taguchi, A. K. W., Allen, J. P., and Woodbury, N. W. (1994) Biochemistry 33, 8089-8100]; and a (3)DeltaG degrees (t) that fits the present data. The assumption that (1)DeltaG degrees (t) = constant is incompatible with our photovo...Continue Reading

References

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Citations

Jul 29, 2005·Photosynthesis Research·Anna DillerJörg Matysik
Sep 23, 2003·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Adalberto Bonincontro, Gianfranco Risuleo
Dec 14, 2011·The Journal of Physical Chemistry. B·Haiyu WangNeal W Woodbury
Mar 11, 2008·Archives of Biochemistry and Biophysics·Francesco Tadini-BuoninsegniKlaus Fendler
Dec 14, 2001·FEBS Letters·M GhoseG Basu
Apr 30, 2003·Biophysical Journal·Zivile KatilieneNeal W Woodbury
Jun 6, 2009·The British Journal of Sociology·Jochem TolsmaLincoln Quillian
Feb 26, 2008·The Journal of Physical Chemistry. a·Joel Gilmore, Ross H McKenzie
Jul 19, 2008·The Journal of Physical Chemistry. B·David N LeBardDmitry V Matyushov

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