Evidence for proteolytic processing and stimulated organelle redistribution of iPLA(2)beta.

Biochimica Et Biophysica Acta
Haowei SongSasanka Ramanadham

Abstract

Over the past decade, important roles for the 84-88kDa Group VIA Ca(2+)-independent phospholipase A(2) (iPLA(2)beta) in various organs have been described. We demonstrated that iPLA(2)beta participates in insulin secretion, insulinoma cells and native pancreatic islets express full-length and truncated isoforms of iPLA(2)beta, and certain stimuli promote perinuclear localization of iPLA(2)beta. To gain a better understanding of its mobilization, iPLA(2)beta was expressed in INS-1 cells as a fusion protein with EGFP, enabling detection of subcellular localization of iPLA(2)beta by monitoring EGFP fluorescence. Cells stably-transfected with fusion protein expressed nearly 5-fold higher catalytic iPLA(2)beta activity than control cells transfected with EGFP cDNA alone, indicating that co-expression of EGFP does not interfere with manifestation of iPLA(2)beta activity. Dual fluorescence monitoring of EGFP and organelle Trackers combined with immunoblotting analyses revealed expression of truncated iPLA(2)beta isoforms in separate subcellular organelles. Exposure to secretagogues and induction of ER stress are known to activate iPLA(2)beta in beta-cells and we find here that these stimuli promote differential localization of iPLA(2)...Continue Reading

Citations

Mar 28, 2013·PloS One·Yuan-Hao HsuEdward A Dennis
May 30, 2015·Journal of Lipid Research·Sasanka RamanadhamXiaoyong Lei
Jan 6, 2012·American Journal of Physiology. Renal Physiology·A PaliegeS Bachmann
Apr 15, 2015·Neuropathology and Applied Neurobiology·Charles Edward ArberSelina Wray
Mar 22, 2014·The Journal of Biological Chemistry·Haowei SongJohn Turk
May 1, 2021·Biomolecules·Tayleur D WhiteSasanka Ramanadham

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