Evidence for resonance optical trapping of individual fluorophore-labeled antibodies using single molecule fluorescence spectroscopy

Journal of the American Chemical Society
Haitao LiDavid Klenerman

Abstract

We report single molecule fluorescence studies of the diffusion of individual multiple fluorophore-labeled antibodies in solution, which show that a trapping potential of about 3.6 k(B)T can be obtained at laser powers below 1 mW with resonant excitation. Individual antibodies can be trapped for up to 140 ms, and bound antibodies can also be used to trap a single virion for up to 1 s. Selective resonance trapping to sort and manipulate fluorophore-labeled biomolecules and complexes may be possible.

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Citations

Dec 1, 2006·The Journal of Physical Chemistry. B·Christopher L KuyperDaniel T Chiu
Jun 15, 2006·Nature Methods·Allison Doerr
May 30, 2007·Molecular BioSystems·Liming Ying
Aug 3, 2013·Physical Chemistry Chemical Physics : PCCP·Tetsuhiro Kudo, Hajime Ishihara
May 15, 2020·Optics Express·Yanan FuXiaocong Yuan
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Sep 15, 2020·Light, Science & Applications·Jungho MunJunsuk Rho
Jan 17, 2008·Chemical Society Reviews·Kishan DholakiaMin Gu
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Aug 19, 2015·The Journal of Physical Chemistry Letters·Tatsuya Shoji, Yasuyuki Tsuboi
Sep 3, 2021·Light, Science & Applications·Jungho MunJunsuk Rho
Apr 18, 2017··Masayuki HoshinaTatsuya Nakai
Sep 9, 2019··Elena I. KurekPeter P. Maksimyak

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