Evidence for role of phospholipase A2 in phosphatidic acid-induced signaling to c-fos serum response element activation

Biochemical and Biophysical Research Communications
B C KimJ H Kim

Abstract

The activity of exogenous phosphatidic acid (PA) to transactivate c-fos serum response element (SRE) was investigated by transient transfection analysis. Incubation of Rat-2 fibroblast cells with exogenous PA caused a stimulation of c-fos SRE-linked luciferase activity in a dose- and time-dependent manner. The SRE stimulation by PA was dramatically reduced by either pre-treatment with mepacrine, an inhibitor of phospholipase A2 (PLA2), or co-transfection with antisense cytosolic phospholipase A2 (cPLA2) oligonucleotide, whereas lysophosphatidic acid (LPA)-induced SRE activation was not affected. Consistent with this specific requirement for PLA2 by PA, the translocation of cPLA2 protein was rapidly induced followed by PA treatment. Together, these results suggest that PLA2, especially cPLA2, plays a critical role in the nuclear signaling cascade of PA in Rat-2 fibroblast cells.

References

Sep 11, 1986·Nature·W H MoolenaarS W de Laat
Jun 2, 1995·The Journal of Biological Chemistry·W H Moolenaar
Jan 27, 1995·Cell·N Divecha, R F Irvine
Feb 1, 1994·Trends in Pharmacological Sciences·M R Boarder
Feb 1, 1993·Current Opinion in Immunology·M M Billah
Mar 6, 1997·Biochemical and Biophysical Research Communications·J H KimG S Lee
Sep 18, 1997·The Biochemical Journal·B C Kim, J H Kim

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Citations

Jul 27, 2001·The Journal of Surgical Research·C C BarnettS A Curley

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