Abstract
The activity of exogenous phosphatidic acid (PA) to transactivate c-fos serum response element (SRE) was investigated by transient transfection analysis. Incubation of Rat-2 fibroblast cells with exogenous PA caused a stimulation of c-fos SRE-linked luciferase activity in a dose- and time-dependent manner. The SRE stimulation by PA was dramatically reduced by either pre-treatment with mepacrine, an inhibitor of phospholipase A2 (PLA2), or co-transfection with antisense cytosolic phospholipase A2 (cPLA2) oligonucleotide, whereas lysophosphatidic acid (LPA)-induced SRE activation was not affected. Consistent with this specific requirement for PLA2 by PA, the translocation of cPLA2 protein was rapidly induced followed by PA treatment. Together, these results suggest that PLA2, especially cPLA2, plays a critical role in the nuclear signaling cascade of PA in Rat-2 fibroblast cells.
References
Jan 1, 1992·The Biochemical Journal·E J van CorvenW H Moolenaar
Feb 20, 1989·Biochimica Et Biophysica Acta·J M Gerrard, P Robinson
Sep 11, 1986·Nature·W H MoolenaarS W de Laat
May 19, 1995·The Journal of Biological Chemistry·M A BalboaP A Insel
Jun 30, 1995·Cell·C S HillR Treisman
Apr 15, 1995·The Biochemical Journal·D T WardV Ohanian
Jun 2, 1995·The Journal of Biological Chemistry·W H Moolenaar
Jan 27, 1995·Cell·N Divecha, R F Irvine
Mar 31, 1994·Biochimica Et Biophysica Acta·T HashizumeT Fujii
Feb 1, 1994·Trends in Pharmacological Sciences·M R Boarder
Dec 1, 1993·The Journal of Cell Biology·K S Ha, J H Exton
Feb 1, 1993·Current Opinion in Immunology·M M Billah
May 24, 1993·FEBS Letters·T SatoT Fujii
Jan 15, 1997·European Journal of Biochemistry·J H Exton
Feb 1, 1997·The Biochemical Journal·Y SnitkoW Cho
Mar 1, 1997·The Biochemical Journal·S A Bauldry, R E Wooten
Mar 6, 1997·Biochemical and Biophysical Research Communications·J H KimG S Lee
Sep 18, 1997·The Biochemical Journal·B C Kim, J H Kim