PMID: 8592067Jan 1, 1996Paper

Evidence for separate control mechanisms at the message, protein, and enzyme activation levels for transglutaminase during calcium-induced differentiation of normal and transformed human keratinocytes

The Journal of Investigative Dermatology
D F GibsonD D Bikle

Abstract

We analyzed the effects of three different calcium concentrations on the RNA and functional protein levels of transglutaminase (TGase) and involucrin (INV) over time in culture. We compared the results in normal human keratinocytes with those in a squamous cell carcinoma, SCC4. The highest calcium concentration (1.2 mM) induced the greatest levels of INV and TGase message, INV protein, and rates of CE formation, but not maximal levels of TGase protein. By examining cytosol and membrane fractions of keratinocytes, we found that after synthesis, TGase protein shifts, under the influence of calcium (both 0.1 mM and 1.2 mM), from the cytosol into the membrane in postconfluent cells. However, only 1.2 mM calcium induced significant amounts of TGase activity. These data indicate that elevated calcium (1.2 mM) achieves the expected induction in keratinocyte differentiation by regulation of not only INV and TGase message levels, but also the translation and activation of TGase protein. Our data suggest that this calcium-induced activation of TGase protein occurs while the protein is anchored in the membrane. In contrast, despite ample INV and TGase message levels within SCC4 cells, these RNA levels are not regulated by calcium or trans...Continue Reading

References

Dec 1, 1990·The Journal of Cell Biology·E Fuchs
Jan 1, 1985·Annals of the New York Academy of Sciences·B A DaleJ D Lonsdale-Eccles
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·F M WattC H O'Neill
Feb 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M J WarholR H Rice
Jun 1, 1985·The Journal of Investigative Dermatology·G K MenonP M Elias
Apr 1, 1994·The Journal of Investigative Dermatology·M DuvicG L Clayman

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Citations

Jul 4, 2003·The Journal of Biological Chemistry·Patricia A LaddDiane S Keeney
May 3, 2005·Archives of Dermatological Research·Sook Jung YunSeung-Chul Lee
May 19, 2000·The Journal of Biological Chemistry·D C NgD D Bikle
Mar 16, 2006·The British Journal of Dermatology·O Pleguezuelos, S Kapas
Nov 1, 1996·Nature Medicine·K A ChoateP A Khavari
Aug 16, 2005·The Journal of Investigative Dermatology·M Ernest DoddWendy B Bollag
Sep 9, 2005·Current Eye Research·Atsuko ToshinoYuichi Ohashi
Nov 12, 2005·Pflügers Archiv : European journal of physiology·Shiwei CaiKenneth T Izutsu
Jul 25, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·L G KömüvesC Largman
Dec 13, 2003·Endocrine Reviews·Stephanie R EdmondsonChristopher J Wraight
Aug 29, 1998·The Journal of Biological Chemistry·Y OdaD D Bikle
Feb 7, 2001·The Journal of Biological Chemistry·K HanleyK R Feingold
Nov 21, 2000·Human Gene Therapy·P A Khavari
May 30, 2001·Molecular and Cellular Endocrinology·D D BikleZ Xie
May 30, 2001·Molecular and Cellular Endocrinology·W Bollinger Bollag, R J Bollag
Apr 16, 1998·The Journal of Investigative Dermatology·K HanleyK R Feingold
Apr 14, 1999·The Journal of Investigative Dermatology·S InuiC Chang
Sep 1, 1999·The Journal of Investigative Dermatology·C L TuD D Bikle
Nov 26, 1999·The Journal of Investigative Dermatology·Y OdaT Mauro
Feb 26, 2000·The Journal of Investigative Dermatology·K HanleyK R Feingold
May 4, 2004·Biochemical and Biophysical Research Communications·Elisa Azuara-LiceagaEsther López-Bayghen
Jul 1, 2004·The Journal of Steroid Biochemistry and Molecular Biology·D D BikleZ Xie
Jun 12, 2004·The Journal of Investigative Dermatology·Matthias SchmuthKenneth R Feingold
Jan 19, 2005·The Journal of Investigative Dermatology·Chia-Ling TuDaniel D Bikle
Nov 25, 2006·The Journal of Investigative Dermatology·Chia-Ling TuDaniel D Bikle
Jan 16, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Yuko OdaDaniel D Bikle
Jan 19, 2007·The Journal of Steroid Biochemistry and Molecular Biology·D BikleY Oda

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