Jul 1, 1975

Evidence for stem cell function of resting bone marrow lymphocytes identified by the complete 3H-thymidine labelling method

Biomedicine / [publiée Pour L'A.A.I.C.I.G.]
D HoelzerR J Haas

Abstract

Resting bone marrow lymphocytes, recongised as small lymphocytes by light microscopy, were labelled by the complete 3H-thymidine labelling technique, enriched by fractionation on a discontinuous albumin gradient and investigated for their stem cell properties by culture in diffusion chambers. Fraction 3, with the highest enrichment of labelled small lymphocytes and slight enrichment of other labelled cells (reticulum and endothelial cells), produced substantial growth, in contrast to fraction 4, with no enrichment of these cells. The number of labelled small lymphocytes per chamber in fraction 3 remained constant or tended to increase. This is assumed to be an indication of some degree of self-replication in the small lymphocyte population. Since, however, their labelling intensity decreased only slowly, it must be further concluded that part of the labelled small lymphocyte population probably remained resting. Some labelled transitional and blast cells appeared before the development of recognisable myelopoietic and erythropoietic precursors and of megakaryocytes, in agreement with the concept that small lymphocytes transfrom to transitional cells during the developmetn of normal haempopiesis.

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Mentioned in this Paper

Bone Marrow Lymphocytes (Lab Test)
Thymidine
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Megakaryocytes
Isotope Labeling, Stable
ALB
Metaphyseal Chondrodysplasia With Cone-shaped Epiphyses, Normal Hair, and Normal Hands
Blast Cell
Small Lymphocyte
Hematopoiesis, Medullary

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