Abstract
A model of the carbohydrate recognition domain CRD, residues 111-245, of hamster galectin-3 has been made using homology modeling and dynamics minimization methods. The model is based on the known x-ray structures of bovine galectin-1 and human galectin-2. The oligosaccharides NeuNAc-alpha2,3-Gal-beta1,4-Glc and GalNAc-alpha1, 3-[Fuc-alpha1,2]-Gal-beta1,4-Glc, known to be specific high-affinity ligands for galectin-3, as well as lactose recognized by all galectins were docked in the galectin-3 CRD model structure and a minimized binding conformation found in each case. These studies indicate a putative extended carbohydrate-binding subsite in the hamster galectin-3 involving Arg139, Glu230, and Ser232 for NeuNAc-alpha2,3-; Arg139 and Glu160 for fucose-alpha1,2-; and Arg139 and Ile141 for GalNAc-alpha1,3- substituents on the primary galactose. Each of these positions is variable within the whole galectin family. Two of these residues, Arg139 and Ser232, were selected for mutagenesis to probe their importance in this newly identified putative subsite. Residue 139 adopts main-chain dihedral angles characteristic of an isolated bridge structural feature, while residue 232 is the C-terminal residue of beta-strand-11, and is followed...Continue Reading
References
Apr 1, 1992·Proteins·A L MorrisJ M Thornton
Jul 20, 1992·Journal of Molecular Biology·M Levitt
Jun 5, 1990·Biochemistry·H AhmedK L Matta
Dec 1, 1991·Glycobiology·A ImbertyS Pérez
Dec 1, 1991·Glycoconjugate Journal·A ImbertyS Pérez
Jun 25, 1991·Carbohydrate Research·J C SolomonT Feizi
Jan 20, 1991·Journal of Molecular Biology·C Lee, S Subbiah
Jul 5, 1990·Journal of Molecular Biology·D G KnellerR Langridge
May 1, 1990·Protein Engineering·C M Wilmot, J M Thornton
Aug 1, 1988·Journal of Biochemistry·Y Ohyama, K Kasai
May 15, 1988·The Biochemical Journal·W M AbbottT Feizi
Nov 5, 1983·Journal of Molecular Biology·M Levitt
Jun 1, 1995·Glycoconjugate Journal·A ImbertyS Pérez
Oct 1, 1994·Glycoconjugate Journal·J Hirabayashi, K Kasai
Oct 1, 1994·Glycoconjugate Journal·A ImbertyS Pérez
May 15, 1995·The Biochemical Journal·R RamkumarS K Podder
Dec 1, 1994·Nature Structural Biology·Y BourneC Cambillau
Oct 1, 1994·Glycobiology·H Ahmed, G R Vasta
Mar 10, 1995·The Journal of Biological Chemistry·M A GittS H Barondes
Nov 11, 1994·Nucleic Acids Research·J D ThompsonT J Gibson
May 1, 1994·Proteins·B Rost, C Sander
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·D I LiaoO Herzberg
Feb 25, 1994·Journal of Molecular Biology·C Lee
Feb 25, 1994·Cell·S H BarondesK Kasai
Feb 1, 1994·Protein Engineering·C Geourjon, G Deléage
Feb 1, 1994·Glycobiology·R C Hughes
May 24, 1994·Biochemistry·T FeiziF T Liu
Nov 1, 1993·Proteins·E C MengI D Kuntz
Aug 1, 1993·Glycobiology·J Hirabayashi, K Kasai
Jan 12, 1993·Biochemistry·S M MassaS H Barondes
Jan 1, 1993·Protein Engineering·G J Barton
Feb 1, 1993·Neurosurgery·I KoyanagiE Theriault
May 31, 1996·The Journal of Biological Chemistry·D SolísT Díaz-Mauriño
Feb 1, 1996·Protein Science : a Publication of the Protein Society·E G Hutchinson, J M Thornton
Dec 1, 1995·Proteins·D Frishman, P Argos
Nov 1, 1996·Journal of Biochemistry·H AhmedG R Vasta
Feb 1, 1996·Protein Engineering·D Frishman, P Argos
Dec 20, 1990·Applied Optics·S Calixto, V Toal
Citations
Sep 11, 2002·Journal of Molecular Biology·Tsuyoshi ShiraiKoji Muramoto
Apr 13, 2000·Biology of the Cell·J C GaudinR C Hughes
Aug 28, 2001·Biochimie·R C Hughes
May 27, 2003·Biochimie·Sulemana BawumiaR Colin Hughes
Dec 2, 1999·Biochimica Et Biophysica Acta·R C Hughes
Feb 14, 2002·Chemical Reviews·Tarun K Dam, C Fred Brewer
Sep 22, 1999·European Journal of Biochemistry·R P Menon, R C Hughes
Sep 3, 2010·The Journal of Biological Chemistry·Emma SalomonssonHakon Leffler
Nov 23, 2000·Glycobiology·E A BarboniR C Hughes
May 19, 2001·Glycobiology·S SassiC M West
Jul 19, 2000·Infection and Immunity·C FradinT Jouault
Apr 4, 2006·Molecular and Cellular Biology·Annick LaganaIvan R Nabi
Jul 13, 2000·Biochemical and Biophysical Research Communications·A L SherwoodE H Holmes
Mar 13, 2014·Expert Review of Molecular Diagnostics·Wouter C MeijersRudolf A de Boer
Nov 29, 2005·FEBS Letters·Erminia BarboniAnna Fiori
Nov 13, 2010·Biochimica Et Biophysica Acta·Mickaël KrzeminskiHans-Joachim Gabius
Nov 17, 2007·Journal of Molecular Biology·Masamichi NagaeRyuichi Kato
Mar 14, 2008·Clinical and Experimental Immunology·E LippertG Rogler
Dec 7, 2006·The American Journal of Pathology·Miliyun G ChiuPaul J D Winyard
Feb 16, 2006·Biochimica Et Biophysica Acta·Jerka DumicMirna Flögel
Oct 27, 2005·Biochemical and Biophysical Research Communications·Pavel LukyanovJosiah Ochieng
Jul 4, 2001·FEBS Letters·K Bachhawat-SikderA Surolia
Dec 8, 2017·Nature Communications·Fausto AlmeidaArturo Casadevall
Feb 7, 2019·Organic & Biomolecular Chemistry·Cecilia Romanò, Stefan Oscarson
Apr 3, 2001·The Journal of Biological Chemistry·R KleeneM Schachner
Aug 26, 1999·Glycobiology·D MercierR Julien
Apr 10, 2003·The Journal of Biological Chemistry·Isabelle PelletierSachiko Sato
Nov 7, 2015·Oncotarget·Maria Claudia VladoiuYves St-Pierre
Sep 26, 2020·Frontiers in Immunology·Diyoly AyonaBenoit Desnues
Jun 5, 2007·The Journal of Biological Chemistry·Hiroko IdeoKatsuko Yamashita
Jul 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Timo K van den BergIrma van Die
Feb 2, 2006·Glycobiology·Holger StalzFranz-Georg Hanisch
Mar 17, 2007·Cancer Research·Mélanie DemersYves St-Pierre
Oct 1, 1998·European Journal of Immunology·V V SwarteG Kraal
Feb 22, 2001·Journal of the American Society of Nephrology : JASN·Simon L BullockAdrian S Woolf
Jun 27, 2018·Journal of the American Society for Mass Spectrometry·Yannick BaschungMichael Przybylski
Jun 20, 2021·Biochimica Et Biophysica Acta. Proteins and Proteomics·Yasuhiro NonakaTakanori Nakamura
Apr 11, 2001·Biochemistry·B BirdsallR C Hughes
Oct 7, 1998·Biochemistry·D D LeonidasK R Acharya
Sep 9, 2006·The Journal of Organic Chemistry·Sébastien FortOle Hindsgaul