PMID: 11900858Mar 20, 2002Paper

Evidence for swelling-induced adenosine and adenine nucleotide release in rat cerebral cortex exposed to monocarboxylate-containing or hypotonic artificial cerebrospinal fluids

Neurochemistry International
J W Phillis, M H O'Regan

Abstract

Recent reports have described a swelling-induced release of adenosine triphosphate (ATP) from a variety of non-nervous system cell types, which may be involved in the regulatory volume decrease (RVD) response. The present study examined the effects of swelling induced by applications of hypotonic or monocarboxylic acid containing artificial cerebrospinal fluid (aCSF) on the release of adenosine nucleotides and adenosine from the in vivo rat cerebral cortex using a cortical cup technique. Hypotonic aCSF (25mM NaCl) elicited a significant increase in adenosine, but not adenine nucleotide, release. Applications of sodium L-lactate, pyruvate, or acetate (all 20mM) evoked increases in adenine nucleotides but not adenosine. D-Lactate (20mM) enhanced adenosine and ATP release. Inhibition of the plasma membrane monocarboxylate transporter with cyano-4-hydroxycinnamate (4-CIN, 2mM) blocked the effects of L-lactate on purine release. These in vivo results demonstrate that osmoregulatory processes in cortical cells evoke an efflux of adenine nucleotides and/or adenosine. In that these purines activate a variety of receptors, it is possible that they may function as autocrine or paracrine signaling agents, facilitating volume regulation an...Continue Reading

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Citations

Aug 24, 2004·Pflügers Archiv : European journal of physiology·Rodrigo FrancoHerminia Pasantes-Morales
Oct 17, 2008·American Journal of Physiology. Cell Physiology·Guangze Li, James E Olson
Dec 17, 2009·Journal of Neuroscience Methods·Christina C PeggChen Xu Wang
Aug 14, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Carole BerthetLorenz Hirt
Mar 7, 2006·Brain Research·Christina R ZamzowFiona E Parkinson
Jul 21, 2005·Neurochemistry International·Alessia MelaniFelicita Pedata
Apr 15, 2005·Neurological Research·Michael O'Regan
Apr 20, 2004·Biology of the Cell·Katleen BraetLuc Leybaert
Feb 27, 2008·Journal of Cellular Physiology·Rodrigo FrancoLenin D Ochoa de la Paz

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