Evidence for synthesis and release of catecholamines by human amniotic epithelial cells

Neuroreport
Mohamed A Elwan, Norio Sakuragawa

Abstract

The present study investigated the presence, possible synthesis and release of catecholamines (CA) by human amniotic epithelial cells (HAEC) using HPLC with electrochemical detection. The presence of CA was indicated by the detection of norepinephrine (NE), dopamine (DA) and its metabolite 3,4-dihydroxyphenylacetic acid (DOPAC) in extracts of cultured HAEC. Incubation of HAE cells in medium supplemented with 1-tyrosine (CA precursor) and tetrahydrobiopterin (tyrosine hydroxylase cofactor) significantly increased the production of catecholamines, suggesting CA synthesis by HAEC. In contrast, pharmacological inhibition of tyrosine hydroxylase by alpha-methyl-p-tyrosine (MPT) significantly reduced CA production, further confirming CA synthesis by HAEC. Catecholamines were also detected in the cell incubation media, demonstrating the ability of HAEC to spontaneously secrete CA. Moreover, incubation of cells with 50 mM K+ for 10 min increased the amount of CA released into the medium. Additionally, the detection of DOPAC, a primary metabolite of DA, in HAEC strongly indicates that these cells contain DA metabolizing enzymes. The present results suggest that HAEC synthesize and release CA. These cells may be a possible candidate for ...Continue Reading

References

Dec 15, 1990·European Journal of Pharmacology·R Dawson, M I Phillips

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Citations

Nov 9, 2000·Journal of Neuroscience Research·S UchidaN Sakuragawa
Nov 13, 2008·Cytotechnology·Marco EvangelistaOrnella Parolini
Feb 1, 2002·European Journal of Pharmacology·Mohamed A Elwan, Norio Sakuragawa
Jun 19, 2003·Neuroscience Letters·Mohamed A ElwanNorio Sakuragawa
Apr 10, 2002·Development, Growth & Differentiation·Satoru KoyanoNorio Sakuragawa
Sep 17, 2011·Tissue Engineering. Part a·Shengli MiChe John Connon
Dec 16, 2004·Transplantation·Marco BailoOrnella Parolini
Aug 27, 2010·Journal of the Royal Society, Interface·Hassan AbdulrazzakPascale V Guillot
Apr 26, 2006·The Tohoku Journal of Experimental Medicine·Hideyuki NakamaNorio Sakuragawa
Mar 26, 2009·Regenerative Medicine·Ornella ParoliniDörthe Schmidt
Jul 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Vanessa SperandioJames B Kaper
Oct 24, 2007·Stem Cell Reviews·Nicol SiegelMarkus Hengstschläger
Nov 9, 2015·Best Practice & Research. Clinical Obstetrics & Gynaecology·Eleni Antoniadou, Anna L David
Feb 23, 2011·Differentiation; Research in Biological Diversity·Silvia Díaz-PradoFrancisco J Blanco
Sep 21, 2004·Journal of Neuroscience Research·Norio SakuragawaYasunobu Yokoyama
Aug 6, 2005·Stem Cells·Toshio MikiStephen C Strom
Aug 21, 2012·Annals of the New York Academy of Sciences·Kavitha SivasubramaniyanHans-Jörg Bühring
Dec 8, 2004·Best Practice & Research. Clinical Obstetrics & Gynaecology·Dario Fauza
Oct 10, 2013·Stem Cell Research·Sittipon Intarapat, Claudio D Stern
Jul 5, 2007·Differentiation; Research in Biological Diversity·Akiva J MarcusIra B Black
Aug 1, 2016·Human Immunology·Stephen C Strom, Roberto Gramignoli
May 15, 2007·Biology of Reproduction·Sivakami IlancheranUrsula Manuelpillai
May 20, 1999·Journal of Child Neurology·N SakuragawaK Kawashima
Jun 9, 2009·Cell Transplantation·Seong Jin YuCesar V Borlongan

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