Evidence for the ability of hippocampal neurons to develop acute tolerance to ethanol in behaving rats

Brain Research
N LudvigP M Bungay

Abstract

The cellular mechanisms underlying acute tolerance to alcohol are unclear. This study aimed to determine whether hippocampal neurons have the ability to develop acute tolerance to alcohol in behaving rats. Intrahippocampal microdialysis was performed in freely behaving rats, and the firing of single neurons in the dialysis area was recorded. The control microdialysis fluid, artificial cerebrospinal fluid (ACSF), was replaced with 1 M ethanol in ACSF for a 30 min period. One hour later, the ethanol perfusion was repeated. To test the functional integrity of the microdialysis probe in situ, each microdialysis session was completed with recording the effect of a 10-20 min perfusion of 500 microM N-methyl-D-aspartate (NMDA). The extracellular concentration profile of ethanol during intrahippocampal microdialysis with 1 M ethanol was estimated in a separate study in anesthetized rats. The ethanol content was measured in tissue slices surrounding the probe with gas chromatography (GC), and the generated data were analyzed with a mathematical model for microdialysis to estimate the concentration of ethanol at the recording site. The predominant effect of the first intrahippocampal microdialysis with ethanol was a decrease in firing ra...Continue Reading

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Citations

Jun 8, 2012·Magnetic Resonance in Chemistry : MRC·Ulf EriksonMarit Aursand
May 2, 2003·British Journal of Pharmacology·Hui-Fang LiJoan J Kendig
Mar 26, 2008·British Journal of Pharmacology·R D MayfieldM A Schuckit
Jul 13, 2005·The Journal of Biological Chemistry·Neesha SuvarnaDorit Ron
Jan 22, 2005·Genes, Brain, and Behavior·H J EdenbergW J McBride
Dec 30, 2014·ACS Chemical Neuroscience·Ashley A Vena, Rueben A Gonzales

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