Evidence for the Adhesive Function of the Exopolysaccharide of Hyphomonas Strain MHS-3 in Its Attachment to Surfaces.

Applied and Environmental Microbiology
E J Quintero, R M Weiner

Abstract

Hyphomonas strain MHS-3 (MHS-3) is a marine procaryote with a biphasic life cycle and which has prosthecate stages that adhere to submerged substrata. We found that adherent forms produced an exopolysaccharide (EPS) capsule that bound Glycine max lectin, Arachis hypogaea lectin, and Bauhinia purpurea lectin (BPA), each having affinity for N-acetyl-d-galactosamine. It also bound the dye Calcofluor. BPA and Calcofluor were tested for the ability to hinder MHS-3 adhesion to glass surfaces; they reduced attachment by >50 and >85%, respectively. Periodate treatment also reduced attachment (by >80%), but pronase treatment did not. Furthermore, an EPS(sup-) variant, Hyphomonas strain MHS-3 rad, did not attach well to surfaces. These results suggest that the MHS-3 EPS capsule is an adhesin.

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Citations

Nov 5, 2005·Applied and Environmental Microbiology·C Eriksson de RezendeR M Weiner
May 26, 2010·FEMS Immunology and Medical Microbiology·Marie GodefroidJean-Jacques Letesson
Mar 1, 2008·Indian Journal of Microbiology·Arundhati Pal, A K Paul
Aug 4, 1998·Applied and Environmental Microbiology·S E Langille, R M Weiner
Aug 30, 2001·Applied and Environmental Microbiology·Y A AnrianyS W Joseph
Dec 16, 1998·Journal of Colloid and Interface Science·N BhosleG G Geesey
Sep 10, 2015·Microbiology Spectrum·Cécile BerneYves V Brun

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