Evidence for the biclonal nature of a Waldenstrom's macroglobulinemia

Clinica Chimica Acta; International Journal of Clinical Chemistry
L BonewaldA C Wang

Abstract

The serum of a patient with Waldenstrom's macroglobulinemia was shown to contain two IgM(kappa) paraproteins of different electrophoretic mobilities on cellulose acetate and immunoelectrophoresis and were designated IgM slow (s) and IgM fast (f). Both existed as high molecular weight polymers and contained a J chain. The two pentameric IgM monoclonal proteins were shown to be completely distinct in carbohydrate composition as determined by gas chromatography and periodic acid-Schiff staining, in molecular weight, by physicochemical properties, peptide mapping, amino-acid composition, NH2-terminal sequence, subgroup of light chains, and by lack of shared idiotypic determinants as determined by hemagglutination inhibition studies. Our study presents strong evidence for the 'bioclonal' nature of this gammopathy. We conclude that both paraproteins are distinct and originated from different B cells.

References

Mar 1, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·G Virella, M C Lechner
Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·C SledgeL Hood
Oct 26, 1967·The New England Journal of Medicine·B J RosenK J Bloch
Apr 14, 1983·Nature·S Tonegawa
Jul 5, 1984·The New England Journal of Medicine·J SklarR Levy
Mar 1, 1980·Molecular Immunology·A C WangL Rivat
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K A KrolickE S Vitetta
Jul 23, 1965·Science·A C Wang, H E Sutton

Citations

Jul 27, 2010·International Journal of Hematology·Kumi NakazakiMineo Kurokawa
Dec 29, 2006·The New England Journal of Medicine·Mandakolathur R MuraliKarin E Finberg
Nov 13, 2020·PloS One·Macarena Boiza-SánchezSocorro María Rodríguez-Pinilla
Apr 4, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jitra KriangkumLinda M Pilarski
Oct 4, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mu HaoFenghuang Zhan

Related Concepts

Chromatography, Gas-Liquid
IgM2
Kappa-Immunoglobulin Subgroup VK-21
Familial Waldenstrom's Macroglobulinaemia
Paraproteins

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