Abstract
Human alpha 2-macroglobulin (alpha 2M), which irreversibly entraps proteinases through a drastic conformational change, has also been reported to bind various cytokines. The meaning of cytokine binding to native and/or transformed alpha 2M molecules is, however, not understood. In an attempt to elucidate this question, we have studied the interaction of radioiodinated recombinant human interleukin-2 (125I-rhIL-2) with native and chymotrypsin (alpha 2M-C)- or methylamine-transformed (alpha 2M-MA) alpha 2M. Our results show that native and alpha 2M-MA are able to bind 125I-rhIL-2, with binding occurring only with the latter in a covalent manner, whereas the labeled cytokine is proteolyzed when incubated with alpha 2M-entrapped chymotrypsin. The degradation of uncomplexed 125I-rhIL-2 has also been observed in the presence of trypsin, whereas 125I-rhIL-2 bound to alpha 2M-MA is protected. Moreover, the proliferative activity of this cytokine on responsive cells is still maintained either with native alpha 2M- or alpha 2M-MA-complexed rhIL-2 in comparison with that observed with the cytokine alone. Our results, which lead us to consider alpha 2M molecules as IL-2-binding proteins, emphasize the possible role of these molecules as im...Continue Reading
References
Sep 8, 1977·Nature·C LargmanM C Geokas
Aug 1, 1979·The Biochemical Journal·A J BarrettC A Sayers
Nov 1, 1979·Analytical Biochemistry·T KureckiM Laskowski
Feb 1, 1992·Archives of Biochemistry and Biophysics·D J WebbS L Gonias
Jan 1, 1992·Electron Microscopy Reviews·E DelainF Van Leuven
Feb 12, 1991·Biochemistry·C T ChuS V Pizzo
Dec 16, 1987·Biochemical and Biophysical Research Communications·F PochonE Delain
Dec 15, 1987·The Biochemical Journal·P B Armstrong, J P Quigley
May 1, 1988·European Journal of Immunology·D Heumann, T L Vischer
Mar 1, 1989·Journal of Neuroscience Research·P H Koo, R W Stach
Jun 13, 1989·Biochimica Et Biophysica Acta·F PochonE Delain
Apr 30, 1985·Biochemical and Biophysical Research Communications·S R FeldmanS V Pizzo
May 27, 1988·Science·K A Smith
Apr 1, 1988·Annals of Internal Medicine·R I FisherK A Margolin
Apr 9, 1987·The New England Journal of Medicine·S A RosenbergJ T Rubin
Jul 15, 1985·The Biochemical Journal·S M LiangD R Thatcher
Oct 30, 1985·Biochemical and Biophysical Research Communications·K J SastryL V Rodriguez
Aug 1, 1973·The Biochemical Journal·A J Barrett, P M Starkey
Jul 1, 1982·The Biochemical Journal·P M Starkey, A J Barrett
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R J RobbV Chowdhry
Jun 29, 1984·Science·A WangD F Mark
Jan 12, 1994·Journal of Immunological Methods·K JamesS F Elliott
May 1, 1993·Endocrinology·J M Vaughan, W W Vale
Aug 10, 1993·Biochemistry·P G Gettins, B C Crews
Apr 1, 1993·Cancer·R O DillmanR Birch
Citations
Jan 29, 2002·Journal of Neuroscience Research·Erin L SkornickaPeter H Koo
Oct 29, 2000·Experimental Parasitology·M de N SoeiroT C Araújo-Jorge
Sep 19, 2003·The Journal of Surgical Research·Tetsuji FujitaSadao Anazawa
Oct 26, 2002·Brain Research. Molecular Brain Research·Feng GaoYansheng Du
Jun 19, 2001·Biochimica Et Biophysica Acta·Q FanR He
Oct 12, 2000·The Biochemical Journal·G J RielyM L Tykocinski
Mar 14, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·T R GarberD J Webb
May 19, 2006·Photomedicine and Laser Surgery·Natalya A Zhevago, Kira A Samoilova
May 15, 1997·Transplantation·G CarayolA Caignard
Dec 12, 2012·PloS One·Sudesh PawariaRobert J Binder
Nov 22, 2007·Experimental Parasitology·E M De SouzaM N C Soeiro
Sep 19, 2006·Journal of the Society for Gynecologic Investigation·Shing-Shun Nelson SiuTze-Kin Lau
Jul 15, 1998·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·X B ZhaoJ M Courtney
Jun 16, 2009·Bulletin of Experimental Biology and Medicine·V U TabakovV V Chestkov
May 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·K ShidaT Seya
Nov 11, 1998·Archives of Biochemistry and Biophysics·M Gunnarsson, P E Jensen
May 11, 2005·Cellular Immunology·Erin L SkornickaPeter H Koo
Feb 24, 2009·Virology·Masmudur M RahmanGrant McFadden
Apr 20, 2010·Micron : the International Research and Review Journal for Microscopy·M M PaivaT C Araújo-Jorge