Evidence for the development of macrophage-like cells in long-term culture of bovine aortic endothelial cells

Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft
K Spanel-BorowskiW C Davis

Abstract

Macrophages are known to be derived from monocytes which proliferate in the bone marrow. The proliferation of monocytes may occur in other places as well. In the present study, we describe the morphological behaviour of macrophage-like cells in endothelial cell cultures obtained from bovine aorta. These cells resembled hemopoietic clones containing progenitor-like cells. Immature and mature macrophage-like cells were rich in acid phosphatase activity, and expressed the CD18 molecule using immunolocalisation. Mature cells contained intracellular lipid droplets. "Actin" globules were apparent only in the peripheral cell areas without lamellipodia or filipodia. At the ultrastructural level, the mature cells were crowded with granules which could be lysosomes, phagolysosomes, or endocytotic vesicles. Multinuclear giant cells which behaved in a different way to the macrophage-like cells were observed. The development and maintenance of macrophage-like cells appears to be dependent on the coculture with endothelial cells. It may signify that endothelial cells are involved in the proliferation of monocytes outside the bone marrow.

References

Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·F AlliotB Pessac
Jul 1, 1989·Ultrastructural Pathology·J M Papadimitriou, R B Ashman
Aug 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J M Robinson
Feb 1, 1987·The Journal of Clinical Investigation·C F Nathan
Jan 1, 1982·Developmental and Comparative Immunology·R A Smith, C A Glomski
Sep 1, 1994·Differentiation; Research in Biological Diversity·K Spanel-BorowskiW F Patton
Mar 1, 1993·Immunology Today·C R Mackay, B A Imhof

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Citations

Aug 7, 2007·International Journal of Hematology·Miyako Bohgaki, Hiromichi Kitaguchi

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