Evidence for the direct involvement of transmembrane region 6 of the lutropin/choriogonadotropin receptor in activating Gs.

The Journal of Biological Chemistry
A N Abell, Deborah L Segaloff

Abstract

The luteinizing hormone/chorionic gonadotropin receptor (LHR) is a heptahelical receptor that interacts primarily with Gs. Previous studies by others have shown that some forms of familial male precocious puberty are associated with mutations of the human LHR in the sixth transmembrane region that result in constitutive activation of the receptor. This study demonstrates that a peptide corresponding to the lower portion of the sixth transmembrane region of the LHR can significantly activate adenylyl cyclase activity. Experiments with membranes derived from wild-type versus cyc- S49 cells demonstrate that the stimulation of cyclase by this peptide is due to an activation of Gs. As such, our data demonstrate a direct role for transmembrane region 6 of the rat LHR in activating Gs and therefore raise the possibility that mutations in transmembrane region 6 of the LHR may directly affect the coupling of the receptor to Gs. Significantly, these data are the first to demonstrate the ability of a transmembrane portion of a G protein-coupled receptor, in the absence of any contributions from an intracellular loop region, to activate a G protein.

References

Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·L LaueG B Cutler
Jun 1, 1993·Endocrine Reviews·D L Segaloff, M Ascoli
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·J LiuJ Wess
Jan 5, 1996·The Journal of Biological Chemistry·E L MartinH E Hamm
Feb 9, 1996·The Journal of Biological Chemistry·D Hill-EubanksM R Brann

❮ Previous
Next ❯

Citations

Sep 7, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Maren ClausRalf Paschke
Jul 9, 1999·Biochimica Et Biophysica Acta·T SchönebergT Gudermann
Mar 14, 2000·Archives of Medical Research·S M Wu, W Y Chan
Jul 20, 1999·Molecular and Cellular Endocrinology·T SchönebergT Gudermann
Sep 28, 2012·Journal of Molecular Endocrinology·Hui Huang, Ya-Xiong Tao
Apr 12, 2002·Endocrine Reviews·Mario AscoliDeborah L Segaloff
Oct 3, 2002·The Journal of Biological Chemistry·Katrin SangkuhlTorsten Schöneberg
Feb 11, 1999·Molecular Endocrinology·A SchulzT Gudermann
Jun 1, 2002·Molecular Endocrinology·Inhae JiTae H Ji

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.