Evidence for the existence of an HP1-mediated subcode within the histone code

Nature Cell Biology
Gwen LomberkRaul Urrutia

Abstract

Currently, the mammalian heterochromatic proteins HP1alpha, HP1beta and the pan-nuclear HP1gamma are considered 'gatekeepers' of methyl-K9-H3-mediated silencing. Understanding how the binding of these proteins to post-translationally modified histones is switched on and off will further our knowledge of how the histone code is modulated. Here, we report that all three HP1 isoforms can be extensively modified, similar to histones, suggesting that the silencing of gene expression may be further regulated beyond the histone code. To assess the potential impact of these modifications, we analysed the phosphorylation of HP1gamma at Ser 83 as a 'model modification'. We demonstrate that P-Ser 83-HP1gamma has an exclusively euchromatic localization, interacts with Ku70 (a regulatory protein involved in multiple nuclear procesess), has impaired silencing activity and serves as a marker for transcription elongation. These observations predict that regulation of silencing by methyl-K9-H3 through modification of mammalian HP1 proteins may be more complex than previously thought and suggests the existence of an HP1-mediated 'silencing subcode' that underlies the instructions of the histone code.

References

Feb 5, 2000·Current Biology : CB·J F Smothers, S Henikoff
Dec 12, 2000·The Journal of Biological Chemistry·K SongI Lee
Dec 31, 2000·The Journal of Biological Chemistry·T ZhaoJ C Eissenberg
Aug 11, 2001·Science·T Jenuwein, C D Allis
Feb 16, 2005·Chromosoma·Leocadia V Paliulis, R Bruce Nicklas
Jul 14, 2005·The Journal of Biological Chemistry·Daniel P MorrisDebra A Schwinn
Jul 23, 2005·Nature·Virginia Gewin

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Citations

Jan 25, 2007·Chromosoma·Katrin HoffmannDonald E Olins
Jul 2, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Catherine Metzler-GuillemainMarie-Genevieve Mattei
Feb 15, 2011·Nature Genetics·Christèle MaisonGeneviève Almouzni
Mar 2, 2011·Nature Structural & Molecular Biology·Violaine Saint-AndréChristian Muchardt
Jul 2, 2010·Reproduction, Fertility, and Development·Corey HeffernanNancy T D'Cruz
Jan 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Emily BernsteinC David Allis
Aug 3, 2010·The Journal of Biological Chemistry·Shuichiro HigoSeiji Takashima
Sep 24, 2008·The Journal of Biological Chemistry·Mohamed El GazzarCharles E McCall
Dec 9, 2010·Molecular & Cellular Proteomics : MCP·Jannie M R DanielsenMichael L Nielsen
Jul 2, 2009·Molecular & Cellular Proteomics : MCP·Gary LeRoyBenjamin A Garcia
Jan 6, 2009·Current Opinion in Gastroenterology·Gwen LomberkRaul Urrutia
Jan 13, 2009·Genes & Development·Atsushi ShimadaYota Murakami
Sep 24, 2008·Molecular and Cellular Biology·Mahito SadaieJun-Ichi Nakayama
Jan 20, 2011·Molecular and Cellular Biology·Kyoko Hiragami-HamadaJun-Ichi Nakayama
Jan 24, 2007·Molecular and Cellular Biology·Rugang ZhangPeter D Adams
Nov 6, 2009·Annual Review of Genetics·Eric I Campos, Danny Reinberg
Jan 17, 2007·Genome Biology·Gwen LomberkRaul Urrutia
Apr 14, 2010·PloS One·Joern BoekeAxel Imhof
Apr 16, 2013·PloS One·Francesca MunariMarkus Zweckstetter
Feb 22, 2011·Journal of Gastrointestinal Cancer·Adrienne GrzendaRaul Urrutia
Feb 15, 2011·Journal of Gastrointestinal Cancer·Gwen A Lomberk
Mar 13, 2014·Nature Communications·Arindam ChakrabortySupriya G Prasanth
Jan 6, 2010·EMBO Molecular Medicine·Leanne De KoningGeneviève Almouzni
Mar 13, 2014·Trends in Cell Biology·Daniele CanzioGeeta J Narlikar
Nov 10, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Randall S Gieni, Michael J Hendzel

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