Evidence for the existence of CCK-producing cells in rat pancreatic islets

Endocrinology
K ShimizuW Y Chey

Abstract

Although the existence of cholecystokinin-like immunoreactivity (CCK-LI) in rat pancreas had been reported previously, it was never clearly demonstrated whether CCK is produced in rat pancreatic islets. The purpose of this study was to elucidate the source of the CCK-LI, the molecular properties of CCK, and the expression of the CCK gene in islet cells. Immunohistochemical studies of rat pancreas were carried out with different rabbit antisera against CCK-8 and CCK-related peptide including N-terminal CCK-33 (1-22) and gastrin-17, and colocalization with known islet hormones including insulin, glucagon, somatostatin, and pancreatic polypeptide was investigated. The major molecular form of CCK in the islets was determined by HPLC. RT-PCR and in situ hybridization were performed to demonstrate the presence of the CCK transcript in the pancreas. CCK-LI was found in the center of the islets, colocalized with insulin in B cells. The major molecular form of CCK in the islets was CCK-8. A 350-nucleotide fragment of PCR-amplified CCK cDNA was detected in the islet as well as the duodenum by RT-PCR. In situ hybridization showed that CCK messenger RNA was located in a large portion of the islets, and this was consistent with the immunohi...Continue Reading

References

Nov 22, 1975·Lancet·J M PolakJ C Thompson
Sep 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L I Larsson
Aug 12, 1976·Nature·L I LarssonR Håkanson
Mar 1, 1991·Molecular Endocrinology·S D KrasinskiA B Leiter
Jul 15, 1987·Biochemical and Biophysical Research Communications·A DutourC Oliver
Jan 1, 1973·Archivum Histologicum Japonicum = Nihon Soshikigaku Kiroku·T Fujita
Jan 1, 1980·Histochemistry·C J GrimmelikhuijzenJ F Rehfeld
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R J DeschenesJ E Dixon
Jul 1, 1996·The Journal of Veterinary Medical Science·Y FuruzawaT Watanabe

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Citations

Mar 5, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kurt BoonenLiliane Schoofs
Aug 29, 2006·Journal of Biochemistry·Hironori UenoOsamu Numata
Jan 30, 2013·Applied Microbiology and Biotechnology·Gustavo MolinaGláucia M Pastore
Dec 20, 2019·Clinical Medicine Insights. Endocrinology and Diabetes·Jens F Rehfeld
Feb 20, 2020·Scientific Reports·Arnaldo Henrique de SouzaDawn Belt Davis
Apr 28, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·C Y IvashchenkoR M Mortensen
May 5, 2010·Advances in Medical Sciences·J Morisset

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