Evidence for the expression of abundant microRNAs in the locust genome

Scientific Reports
Yan-Li WangLe Kang

Abstract

Substantial accumulation of neutral sequences accounts for genome size expansion in animal genomes. Numerous novel microRNAs (miRNAs), which evolve in a birth and death manner, are considered evolutionary neutral sequences. The migratory locust is an ideal model to determine whether large genomes contain abundant neutral miRNAs because of its large genome size. A total of 833 miRNAs were discovered, and several miRNAs were randomly chosen for validation by Northern blot and RIP-qPCR. Three additional verification methods, namely, processing-dependent methods of miRNA biogenesis using RNAi, evolutionary comparison with closely related species, and evidence supported by tissue-specific expression, were applied to provide compelling results that support the authenticity of locust miRNAs. We observed that abundant local duplication events of miRNAs, which were unique in locusts compared with those in other insects with small genome sizes, may be responsible for the substantial acquisition of miRNAs in locusts. Together, multiple evidence showed that the locust genome experienced a burst of miRNA acquisition, suggesting that genome size expansion may have considerable influences of miRNA innovation. These results provide new insight...Continue Reading

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Citations

Oct 27, 2016·BMC Genomics·Alicja PacholewskaVincent Gerber
Nov 25, 2016·Scientific Reports·Guillem YllaXavier Belles
Jun 10, 2020·Neotropical Entomology·C Villagra, D Frías-Lasserre
Nov 5, 2016·Annual Review of Entomology·Xavier Belles
Aug 16, 2018·Journal of Neurophysiology·Hongxia WangFabrizio Gabbiani
Jun 24, 2021·F1000Research·Heleen VerlindenJozef Vanden Broeck

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Datasets Mentioned

BETA
SRP062155
AVCP000000000

Methods Mentioned

BETA
immunoprecipitation
PCR
in
PCRs
immunoprecipitations

Software Mentioned

Bioconductor R package
MapMi
GLSEARCH
BLAST
edgeR
Cutadapt
FASTA
BLASTN
RepeatMasker
custom Perl scripts

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