PMID: 9434095Jan 20, 1998Paper

Evidence for the involvement of calpain in cataractogenesis in Shumiya cataract rat (SCR)

Biochimica Et Biophysica Acta
M InomataS Shumiya

Abstract

The Shumiya cataract rat (SCR) is a hereditary cataract model in which lens opacity appears spontaneously in the nuclear and perinuclear portions at 11-12 weeks of age. It was found that the proteolysis of some crystallins and cytoskeletal proteins is significantly enhanced in cataractous SCR lenses. The calcium concentrations in cataractous lenses rise markedly with age as compared with control lenses and the autolytic product of calpain is also detected in cataractous lenses. In order to provide direct evidence for the involvement of calpain in the proteolytic modification of lens proteins, we developed antibodies exclusively specific to the proteolytic products of some lens proteins produced by the action of calpain and analyzed their degradation during cataractogenesis in SCR by Western blotting and immunohistochemical staining. The results demonstrate that calpain participates in the proteolytic modification of lens proteins, at least alpha-crystallin (A and B chain), betaB1-crystallin, and alpha-fodrin. The proteolytic products formed by the action of calpain on these proteins are detected in cataractous lenses of SCR as young as 8 weeks of age and accumulate with age. It was also found that betaB1-crystallin, originally ...Continue Reading

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Citations

Mar 1, 2008·Journal of Ocular Biology, Diseases, and Informatics·T Naga RajuK Lavanya
Apr 10, 2009·American Journal of Physiology. Cell Physiology·Mauricio A RetamalLuis Reuss
Jan 28, 2006·The Journal of Clinical Investigation·Masayuki MoriSeigo Shumiya
Jun 5, 2012·Journal of Molecular and Cellular Cardiology·Kaori Shintani-IshidaKen-Ichi Yoshida
Jan 11, 2000·Eye·J I ClarkJ M Clark
Jan 11, 2008·American Journal of Physiology. Cell Physiology·Eri KuboDhirendra P Singh
Feb 21, 2014·The Journal of Biological Chemistry·Puttur SanthoshkumarK Krishna Sharma
Jun 27, 1998·Biochemical and Biophysical Research Communications·E Carafoli, M Molinari

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