Evidence for the involvement of ecto-5'-nucleotidase (CD73) in drug resistance

International Journal of Cancer. Journal International Du Cancer
P UjházyE Mihich

Abstract

Increased ecto-5'-nucleotidase (ecto-5'NT) protein expression in several multidrug-resistant (MDR) cell lines, documented previously by our group, suggests that this enzyme is involved in drug resistance. Here, Northern blot analysis of selected cell lines and their MDR variants positively correlated ecto-5'NT protein with its mRNA expression. An inhibitor of ecto-5'NT enzymatic activity, alpha,beta-methyleneadenosine 5'-diphosphate (AMP-CP), was used to determine if functionally active enzyme had a role in drug resistance. AMP-CP (0.3 mM) reversed the resistance of ecto-5'NT-positive MDR cells (MCF7/A6, L1210/A) to doxorubicin, whereas it did not affect the doxorubicin sensitivity of the ecto-5'NT-negative parental cell lines or that of 2 ecto-5'NT-negative MDR cell lines (HL60/VCR and A2780/DX5). Furthermore, AMP-CP increased rhodamine uptake and inhibited rhodamine efflux from ecto-5'NT-positive MDR cells without affecting ecto-5'NT-negative MDR cells. The presence of exogenous adenosine (0.5 microM) circumvented AMP-CP-induced inhibition of rhodamine efflux from EL4/ADM cells. AMP-CP inhibited the growth of the ecto-5'NT-positive L1210/A MDR cells but had no effect on the growth of the parental cell line. Determination of i...Continue Reading

References

Jul 15, 1992·The Biochemical Journal·H Zimmermann
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·E H AbrahamH F Cantiello
Aug 1, 1994·International Journal of Cancer. Journal International Du Cancer·K Mehta
Oct 1, 1994·International Journal of Cancer. Journal International Du Cancer·P UjházyM J Ehrke
Aug 3, 1994·Biochemical Pharmacology·M K RitkeJ C Yalowich

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Citations

Apr 13, 2000·The Plant Cell·C ThomasS J Roux
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·John StaggMark J Smyth
Jul 19, 2008·Molecular and Cellular Biochemistry·Luci BavarescoAna Maria Oliveira Battastini
Apr 29, 1998·Immunological Reviews·R RestaL F Thompson
Jun 22, 2012·Oncoimmunology·Bin Zhang
Jul 27, 2012·Journal of Cellular Physiology·Claudia QuezadaRody San Martín
Sep 11, 2014·Journal of Extracellular Vesicles·Deep PokharelMary Bebawy
Dec 18, 2018·Expert Opinion on Therapeutic Targets·Ghasem GhalamfarsaFarhad Jadidi-Niaragh
Jul 11, 2006·Molecular and Cellular Biochemistry·Alcira A BernardoVivian M Rumjanek
Jan 30, 2019·Journal of Cellular Physiology·Anvar SoleimaniMahmoud Reza Jaafari
Aug 3, 2007·Journal of Cancer Research and Clinical Oncology·Li WangPing Zhou
Sep 5, 2009·Investigational New Drugs·Umadevi SubramanianA J Vanisree
Jul 28, 2010·Oncogene·J Stagg, M J Smyth
Jun 2, 2012·British Journal of Cancer·C Lo NigroT Crook
Jan 28, 2014·Purinergic Signalling·Liliana RockenbachAna Maria Oliveira Battastini
Aug 16, 2014·BioMed Research International·Zhao-wei GaoHui-zhong Zhang
Dec 8, 2015·The Journal of Clinical Investigation·Jessica L BowserRussell R Broaddus
Aug 23, 2011·Molecular and Cellular Biochemistry·Angélica Regina CappellariAna Maria Oliveira Battastini
Jul 17, 2008·Molecular and Cellular Biochemistry·Sundaram JaganThiruvengadam Devaki
Aug 20, 2015·Journal of Cancer Research and Clinical Oncology·Paloma Silva de SouzaRaquel Ciuvalschi Maia
Jun 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrey MikhailovJohn E Eriksson
Jun 22, 2019·Immunotherapy·Siqi ChenBin Zhang
Oct 27, 2016·Chemical Biology & Drug Design·Pervaiz Ali ChannarJamshed Iqbal
Nov 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Franciele Aline BruinsmannFabio Sonvico
May 1, 2020·Frontiers in Immunology·Jerry B HarveyJessica L Bowser
Jan 24, 2021·Clinical and Experimental Medicine·Samaneh ArabSamira Asgharzade
Jan 13, 2021·Cancers·Haitang YangSean R R Hall
Feb 12, 2010·Journal of Medicinal Chemistry·Younis BaqiChrista E Müller
Sep 29, 2000·Pharmacology & Therapeutics·J Spychala
Aug 2, 2003·Molecular and Cellular Endocrinology·M R WinkA M O Battastini
Jun 21, 2005·Pharmacology & Therapeutics·Sally Anne HunsuckerJozef Spychala

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