Evidence for the predominance of a single tet(M) gene sequence type in tetracycline-resistant Ureaplasma parvum and Mycoplasma hominis isolates from Tunisian patients

Journal of Medical Microbiology
Boutheina Ben Abdelmoumen MardassiBéhija Mlik

Abstract

Resistance to tetracyclines in genital mycoplasmas is due mainly to acquisition of the tet(M) determinant, which is frequently associated with conjugative transposon elements of the Tn916/Tn1545 family. The aim of the present work was to evaluate the prevalence of tet(M) in Tunisian isolates and to gain an insight into its origin and evolution. Twenty Ureaplasma parvum, two Ureaplasma urealyticum and 48 Mycoplasma hominis isolates, recovered from Tunisian patients with urogenital and infertility disorders, were evaluated for their resistance to tetracyclines and interrogated by PCR amplification for the presence of tet(M) and int-Tn, the gene encoding the integrase of Tn916/Tn1545-like transposons. The resistance rates to tetracyclines were 22.72 and 25.0 % among U. parvum and M. hominis isolates, respectively, with high-level resistance observed in 11 of the 12 resistant M. hominis isolates. All resistant isolates harboured both tet(M) and int-Tn sequences. Nucleotide sequence analysis of the tet(M) amplicon revealed a unique sequence shared by all tetracycline-resistant clinical isolates of both species. Molecular typing indicated that the tetracycline-resistant U. parvum and M. hominis isolates were not clonal. Taken togethe...Continue Reading

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Citations

Apr 1, 2014·BMC Infectious Diseases·Mathys J RedelinghuysMarleen M Kock
Jan 24, 2015·Journal of Pathogens·Sunčanica Ljubin-Sternak, Tomislav Meštrović
Nov 1, 2016·The Journal of Antimicrobial Chemotherapy·M L Beeton, O B Spiller
Mar 2, 2019·The Journal of International Medical Research·Brenda Maldonado-ArriagaJuan Antonio Suárez-Cuenca
Jul 10, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Lihong ZhaoShuping Zhao
Jun 5, 2019·International Journal of Molecular Sciences·Mi-Sun KangSang-Phil Hong
Jul 17, 2020·Infection and Drug Resistance·Safa BoujemaaBoutheina Ben Abdelmoumen Mardassi
Jul 18, 2019·The Journal of Obstetrics and Gynaecology Research·Ourlad Alzeus G Tantengco, Itaru Yanagihara
Jan 31, 2020·Antimicrobial Resistance and Infection Control·Safa BoujemaaBoutheina Ben Abdelmoumen Mardassi
Jul 30, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Daniel J MorrisOwen B Spiller
Jul 22, 2015·Antimicrobial Agents and Chemotherapy·Michael J Calcutt, Mark F Foecking
Feb 1, 2020·Antimicrobial Resistance and Infection Control·Safa BoujemaaBoutheina Ben Abdelmoumen Mardassi

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