Evidence for the presence in calf thymus of a peptidic factor controlling DNA transcription in vitro.

Biochimica Et Biophysica Acta
G GianfranceschiL Guglielmi

Abstract

A thymic factor causes a strong inhibition of the DNA-directed RNA polymerase reaction in vitro. The active factor was isolated from aqueous ultrafiltered thymus extracts and purified by means of chromatography on DEAE-cellulose and then on Dowex 50 WX2. The purified thymic factor was characterized as a peptide of low molecular weight (less than 5000). The biological activity of the thymic factor cannot be attributed to the presence of a nuclease or of a histone fragment. The RNA synthesis is controlled by this factor by means of electrostatic interactions between the peptide compound and DNA. Inhibitory activity on RNA synthesis was absent from kidney extracts.

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Citations

Sep 1, 1976·Molecular Biology Reports·G L GianfranceschiL Guglielmi
Feb 1, 1991·Molecular Biology Reports·F FeliciG L Gianfranceschi
Mar 31, 1980·Molecular Biology Reports·G L GianfranceschiL Guglielmi
Jul 31, 1980·Molecular Biology Reports·M Hillar, J Przyjemski
Jul 1, 2000·Journal of the American Aging Association·A Zaia, L Piantanelli
Nov 1, 1983·Mechanisms of Ageing and Development·D AmiciG L Gianfranceschi
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D AmiciG L Gianfranceschi
May 1, 1980·Archives internationales de physiologie et de biochimie·F PetrelliD Barra
Apr 7, 2015·International Journal of Biological Macromolecules·Andrey Y SolovyevYury A Skorik
Nov 30, 1982·Biochimica Et Biophysica Acta·G L GianfranceschiD Amici
Jan 1, 1992·Archives of Gerontology and Geriatrics·G RossoliniG L Gianfranceschi
Dec 1, 1977·Molecular Biology Reports·G L GianfranceschiR Petruzzelli
Nov 25, 1994·Annals of the New York Academy of Sciences·A BassoG L Gianfranceschi
Feb 3, 1977·Biochimica Et Biophysica Acta·O AntonoglouA Trakatellis
Nov 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Vladimir Khatskelevich KhavinsonAnastasiia Romanovna Ilina

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