Evidence for the presence of a Hg-inhibitable water-permeability pathway and aquaporin 1 in A5 salivary epithelial cells

Journal of Cellular Physiology
K W LazowskiB J Baum

Abstract

The molecular basis of water-permeability in salivary and other exocrine glands is not understood. We have examined two well-studied salivary epithelial cell lines for evidence of a Hg-inhibitable water-permeability pathway. A5 and HSG cells are derived from rat and human submandibular glands, respectively. Only A5 cells demonstrated such a pathway. The rate of A5 cell osmotic shrinkage was inhibited about fivefold in the presence of 300 microM HgCl2. To determine if this activity was associated with the expression of the prototypical water channel (aquaporin, AQP) AQP1, we used three separate experimental approaches; Northern analysis and reverse transcription-polymerase chain reaction (RT-PCR) analysis of isolated mRNA, and Western analysis of cell membranes. All three methods yielded positive results with A5 cells and negative results with HSG cells. The approximately 800 bp product of RT-PCR was analyzed further by sequencing and restriction enzyme digestion. The results were consistent with the previously reported coding region sequence for rat kidney AQP1. The aggregate data demonstrate that marked differences in water-permeability and water channel expression exist in these two salivary epithelial cell lines.

References

Nov 16, 1992·Biochemical and Biophysical Research Communications·P M DeenC H Van Os
Jan 1, 1992·Annual Review of Physiology·A S Verkman
Sep 1, 1992·Molecular and Cellular Biology·A LanahanD Nathans
Sep 1, 1991·The American Journal of Physiology·J P Dehaye, R J Turner
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G M Preston, P Agre
Sep 1, 1973·The American Journal of Physiology·J A MangosS N Arvanitakis
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·M A Knepper
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·J S JungP Agre
Oct 1, 1994·Pflügers Archiv : European journal of physiology·J LiB J Baum
Sep 20, 1993·Annals of the New York Academy of Sciences·R J Turner
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S NielsenP Agre

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Citations

Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·C DelporteB J Baum
Mar 16, 2006·Biochimica Et Biophysica Acta·Christine Delporte, Serge Steinfeld
May 20, 2006·Cognition·Daniele Amati, Tim Shallice
Jul 4, 2013·The Biochemical Journal·Anna FrickSusanna Törnroth-Horsefield

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