PMID: 9420841Jan 1, 1995Paper

Evidence for the presence of AH 13205-sensitive EP2-prostanoid receptors in the pregnant baboon but not in the pregnant sheep myometrium near term

Journal of the Society for Gynecologic Investigation
R Garcia-VillarP W Nathanielsz

Abstract

Our purposes were to assess the effects of prostaglandin (PG) E2 and PGF2 alpha on myometrial contractility in pregnant sheep and baboons in an in vitro superfusion study, and to characterize further the PGE-sensitive (EP) receptor subtype involved in the myometrial response to PGE2 by using the selective prostanoid EP2 agonist AH 13205. Strip preparations of uterine muscle from 15 sheep (107-145 days' gestational age) and ten baboons (158-185 days' gestation) were studied. Cumulative concentration-response curves (CRC) were constructed to oxytocin (4.2 pmol/L to 0.42 mumol/L, PGE2 (0.1 nmol/L to 1 mumol/L), and PGF2 alpha (1 nmol/L to 100 mumol/L), and 50% effective concentration (EC50) values (mean and 95% confidence interval) were calculated. We also tested the hypothesis that PGE2-induced myometrial relaxation in pregnant baboons could be mediated by EP2-prostanoid receptors. Myometrial strips were stimulated by oxytocin (0.42 nmol/L), and CRCs to the EP2-agonist AH 13205 (0.1 nmol/L to 10 mumol/L) were constructed. Prostaglandin F2 alpha stimulated myometrial activity in a concentration-related fashion in all preparations from both sheep and baboons. The EC50 in the sheep myometrium for PGF2 alpha (52 nmol/L, 95% confidenc...Continue Reading

Citations

Dec 20, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Peter W NathanielszWen Wu
Mar 4, 2003·Prostaglandins & Other Lipid Mediators·Cecilia LyonsMichael P Nageotte
Nov 1, 2001·Journal of Pharmacological and Toxicological Methods·D J Crankshaw
Sep 10, 1998·American Journal of Obstetrics and Gynecology·A E PiteraP W Nathanielsz
Mar 1, 1997·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·C R Taylor

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