Evidence for the presence of thioltransferase in the lens

Experimental Eye Research
N Raghavachari, M F Lou

Abstract

Thioltransferase (TTase) activity was identified and partially purified from the ocular tissue for the first time. The enzyme activity depended on the presence of reduced glutathione (GSH), glutathione reductase (GR) and NADPH to reduce the disulfide bond in a synthetic substrate, hydroxyl ethyl disulfide (HEDS). Maximum activity was obtained in a pH 7.4 phosphate buffer at 30 degrees C. This enzyme distinguishes from other reducing enzymes such as thioredoxin that do not require GSH and GR for their catalytic activity. It also differs from the 52 kDa enzyme, protein disulfide isomerase by its smaller molecular size and its stability against heat treatment. TTase activity was higher in the epithelial layer but distributed evenly in the rest of the lens also, TTase showed similar activity in the lenses obtained from rats, pigs, bovine, guinea pigs, chick embryos and humans. The molecular weight of this enzyme was estimated to be 11.5 kDa on a SDS-PAGE system. Western blot analysis showed the protein reacted positively to the antibody raised by the purified pig liver TTase. Similarly the antibody raised by the partially purified lens enzyme reacted positively with the purified pig lever TTase. The presence of TTase in the lens wa...Continue Reading

Citations

Aug 22, 2009·Virus Genes·Lisa A FitzgeraldJames L Van Etten
Jun 3, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Xun LiGeorge J Rozanski
Sep 27, 2012·Investigative Ophthalmology & Visual Science·Jie ZhangMarjorie F Lou
Sep 26, 2002·Journal of Biochemistry and Molecular Biology·Minjung KimDaemyung Kim
Aug 12, 2009·Experimental Eye Research·Linda M MeyerPer Söderberg
May 10, 2000·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·M F Lou
Nov 12, 1998·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·J E DickersonM F Lou
Nov 13, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Xun LiGeorge J Rozanski
Jun 7, 2019·Metallomics : Integrated Biometal Science·Valeria ScalconMaria Pia Rigobello
Jan 15, 2002·The Journal of Biological Chemistry·Iraimoudi S AyeneCameron J Koch
Sep 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kuiyi Xing, Marjorie F Lou
Jun 9, 2001·The Journal of Biological Chemistry·V N GladyshevM F Lou
Oct 4, 2008·Antioxidants & Redox Signaling·Viviana M Berthoud, Eric C Beyer
Nov 5, 2014·The Journal of Biological Chemistry·Hongli WuMarjorie F Lou
Jan 23, 2020·Pharmaceuticals·Segewkal H HeruyeCatherine A Opere
Jun 14, 2000·Experimental Eye Research·F QiaoM F Lou
Feb 8, 2000·The International Journal of Biochemistry & Cell Biology·N NodaT Terada
Aug 2, 2003·Progress in Retinal and Eye Research·Marjorie F Lou

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