Evidence for the role of angiotensin II biosynthesis in the rat internal anal sphincter tone

Gastroenterology
Márcio A F de GodoySatish Rattan

Abstract

The internal anal sphincter tone is important for anorectal continence. This study examined the role of angiotensin II as a neurohumoral signal for the myogenic tone in the internal anal sphincter. We determined the effect of angiotensin I, II, III, and IV and angiotensin-(1-7) on the basal tone of the rat internal anal sphincter smooth muscle before and after selective receptor antagonists and biosynthesis inhibitors. Selective pharmacological tools used were losartan (for the AT(1) receptor), PD123,319 (for AT(2)), A-779 [for angiotensin-(1-7)], captopril (for angiotensin-converting enzyme), and amastatin (for aminopeptidases A and N). Angiotensins were measured by using high-performance liquid chromatography/UV. Western blot studies were used to determine AT(1) and AT(2) receptors, ACE, and aminopeptidases A and N. Angiotensin I, II, and III produced concentration-dependent contraction in the internal anal sphincter mediated by AT(1) receptors. However, in the higher concentrations (from 100 nM to 10 microM), angiotensin II showed an inhibitory effect via AT(2) receptors. Captopril (1 microM) inhibited the biosynthesis of angiotensin II in the internal anal sphincter, antagonized the contractile effects of angiotensin I, and...Continue Reading

References

Jan 1, 1978·Biochemical Pharmacology·M A Devynck, P Meyer
Jun 26, 1992·Brain Research·J W HardingJ W Wright
Jan 1, 1989·Pharmacotherapy·A L HumeS E Lauerman
Jan 1, 1982·Physiological Reviews·A J Kenny, S Maroux
Jan 4, 1998·Kidney International·R Ardaillou, D Chansel
Feb 14, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ya-Ping FanSatish Rattan
Jul 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Weibiao CaoPiero Biancani
Jul 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Rajinder N PuriSatish Rattan
Sep 18, 2002·The Journal of Pharmacology and Experimental Therapeutics·Márcio A F de Godoy, Ana M de Oliveira
Oct 29, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Adriana Pelegrini-da-SilvaAntonio R Martins

❮ Previous
Next ❯

Citations

Jan 14, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Satish Rattan, Jagmohan Singh
Dec 6, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Márcio A F de GodoySatish Rattan
Feb 18, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Márcio A F de GodoySatish Rattan
Apr 19, 2011·Trends in Pharmacological Sciences·Márcio A F de Godoy, Satish Rattan
Jan 24, 2007·Gastroenterology·Anna CasselbrantLars Fändriks
Apr 20, 2005·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·S Rattan
Oct 14, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·A OpazoD Gallego
Dec 14, 2011·International Journal of Hypertension·Marcio Augusto Fressatto de GodoySatish Rattan
Mar 25, 2008·Journal of Pharmacological Sciences·Leposava GrbovicSrdan Pesic
Oct 13, 2015·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·Shahram AlaRoja Qobadighadikolaei
Dec 10, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Satish Rattan
Jan 29, 2014·Journal of the Renin-angiotensin-aldosterone System : JRAAS·A CasselbrantH Lönroth
Oct 29, 2009·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Lars Fändriks
Jan 29, 2009·The American Journal of Gastroenterology·Osvaldo BorrelliSalvatore Cucchiara
Aug 7, 2007·Techniques in Coloproctology·E E Collins, J N Lund
Jul 16, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Márcio A F De Godoy, Satish Rattan
Aug 25, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Márcio A F de Godoy, Satish Rattan
Sep 21, 2006·The Journal of Pharmacology and Experimental Therapeutics·Márcio A F de Godoy, Satish Rattan
Jul 19, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Chadalavada Vijay KrishnaSatish Rattan
Apr 18, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Satish RattanBenjamin Phillips
Nov 5, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Arjun SinghSatish Rattan
Jun 3, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Arjun Singh, Satish Rattan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.