PMID: 8001263Dec 1, 1994

Evidence for the simultaneous expression of alternatively spliced alkylpurine N-glycosylase transcripts in human tissues and cells

Carcinogenesis
A PendleburyJ A Rafferty

Abstract

We have isolated a novel human alkylpurine N-glycosylase (APNG) cDNA from a placental library by screening with an oligonucleotide based on the published sequence of the human liver cDNA encoding this protein. The nucleotide sequences of the two cDNAs were essentially identical, but the 5' untranslated region of the new sequence was truncated and the 5'-terminal 92 nucleotides of the novel cDNA were different, indicating the possibility of alternative transcripts. This region included a portion of the open reading frame, so that the predicted protein was truncated and the seven N-terminal amino acids differed from the published sequence for APNG. PCR amplification of reverse transcribed mRNA, using 5' primers unique to the two cDNAs and a common 3' primer showed that the alternative transcripts can be co-expressed in the same cells and tissues.

References

Aug 10, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M D WyattLeona D Samson
Aug 27, 2005·Mitochondrion·Jakob Christian MuellerThomas Meitinger
Nov 26, 2003·Mutation Research·Laurent GrosMurat K Saparbaev
Nov 11, 2008·Mutation Research·Shareen H DoakGareth J S Jenkins
Feb 23, 2010·Osteoarthritis and Cartilage·T J HeyseM D Schofer
Dec 17, 2002·Oncogene·Laurent GrosJacques Laval
Feb 6, 2004·The Journal of Biological Chemistry·Laurent GrosMurat K Saparbaev
Sep 1, 2016·BMC Molecular Biology·Lijian YuMichael R Volkert

Related Concepts

MPG gene
MPG wt Allele
Amino Acids, I.V. solution additive
Tissue Specificity
Placenta
5' Untranslated Regions
Homologous Sequences, Amino Acid
Gene Amplification Technique
Gene Expression Regulation, Enzymologic
DNA-3-methyladenine glycosidase II

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