Evidence for trans-cis isomerization of the p-coumaric acid chromophore as the photochemical basis of the photocycle of photoactive yellow protein.

FEBS Letters
R KortKlaas J Hellingwerf

Abstract

Analysis of the chromophore p-coumaric acid, extracted from the ground state and the long-lived blue-shifted photocycle intermediate of photoactive yellow protein, shows that the chromophore is reversibly converted from the trans to the cis configuration, while progressing through the photocycle. The detection of the trans and cis isomers was carried out by high performance capillary zone electrophoresis and further substantiated by 1H NMR spectroscopy. The data presented here establish the photo-isomerization of the vinyl double bond in the chromophore as the photochemical basis for the photocycle of photoactive yellow protein, a eubacterial photosensory protein. A similar isomerization process occurs in the structurally very different sensory rhodopsins, offering an explanation for the strong spectroscopic similarities between photoactive yellow protein and the sensory rhodopsins. This is the first demonstration of light-induced isomerization of a chromophore double bond as the photochemical basis for photosensing in the domain of Bacteria.

References

Aug 11, 1989·Journal of Chromatography·B L KargerA Guttman
Jul 1, 1996·Biophysical Journal·M E Van BrederodeK J Hellingwerf
Nov 15, 1985·Science·E GassmannR N Zare

❮ Previous
Next ❯

Citations

Apr 25, 2012·Journal of Natural Medicines·Naohiro OshimaFumiyuki Kiuchi
Mar 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andrew F PhilipWouter D Hoff
Mar 30, 2000·Protein Science : a Publication of the Protein Society·D M van AaltenL Joshua-Tor
Apr 15, 2010·The Journal of Chemical Physics·D C Tranca, A A Neufeld
Jan 26, 2011·The Journal of Chemical Physics·Dmitry ZuevAnna I Krylov
Dec 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Rene A NomeNorbert F Scherer
Jul 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jason Ming ZhaoWouter D Hoff
Aug 9, 2013·Bioscience, Biotechnology, and Biochemistry·Takahiko MitaniYoshihiko Ozaki
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·R CordfunkeK J Hellingwerf
Jul 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·B C LeeW D Hoff
Feb 24, 2010·Phytochemistry·Kjirsten A WaymanNigel B Perry
Aug 14, 2015·Photochemistry and Photobiology·Akihiro ShiraiTakeshi Omasa
Feb 28, 2006·Biophysical Journal·Javaid Shahbaz KhanMasahide Terazima
Jun 3, 2008·Photochemistry and Photobiology·Miki HarigaiYasushi Imamoto
Mar 24, 2016·The Journal of Physical Chemistry Letters·Tim Stauch, Andreas Dreuw
Apr 26, 2015·The Journal of Physical Chemistry. a·F Fernández García-PrietoM E Martín
Jun 15, 1997·Structure·I Schlichting, J Berendzen
Feb 22, 2005·Biophysical Journal·Jocelyne VreedePeter G Bolhuis
Jul 26, 2005·Biophysical Journal·I B NielsenL H Andersen
Mar 26, 2004·Biophysical Journal·Delmar S LarsenRienk van Grondelle
Sep 3, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Feng GaoWenbin Zeng
Oct 22, 2016·Chemical Reviews·Tim Stauch, Andreas Dreuw
Nov 10, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·María Torres-NaranjoVladimir Morocho
Jan 11, 2017·Journal of Chemical Theory and Computation·Francisco F García-PrietoM Elena Martín
Mar 30, 2017·Journal of Computational Chemistry·Kenji HiranoShigeyoshi Sakaki
Jul 17, 2015·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Norio HamadaRyosuke Nakamura
Mar 31, 2016·Physical Chemistry Chemical Physics : PCCP·Michael A ParkesHelen H Fielding
Aug 30, 2003·Photochemistry and Photobiology·Remco KortMichael Wulff

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.