Evidence for transcriptional regulation of plastid photosynthesis genes in Arabidopsis thaliana roots

Plant Physiology
K IsonoH Kobayashi

Abstract

Mechanisms underlying suppressed levels of transcripts for plastid photosynthesis genes in nongreen tissues such as roots and calli were analyzed in Arabidopsis thaliana, a plant suitable for further genetic dissection. A region encoding promoters of rbcL, the gene encoding the large subunit of ribulose-1,5-biphosphate carboxylase/oxygenase, and the atpB/E operon for the beta and epsilon subunits of coupling factor one were cloned and sequenced. Transcripts for rbcL, atpB/E, and psbA, the gene for the D1 protein in the photosystem II reaction center, were barely detectable in roots of A. thaliana, whereas 16S rRNA was detected at a low level. The run-on transcription experiment revealed that expression of rbcL, atpB/E, and psbA was regulated at transcription. The copy number of plastid DNA in roots was one-fifth that in green leaves on the basis of total cellular DNA, suggesting that in the latter the DNA copy-number regulation also exists in plastid gene expression. Digestion of DNA with methyl-sensitive and -insensitive isoschizomeric endonucleases and subsequent polymerase chain reaction, as well as in vitro transcription of plastid DNAs with Escherichia coli RNA polymerase, resulted in no evidence of regulation by DNA modif...Continue Reading

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Citations

Oct 14, 2004·Functional & Integrative Genomics·Daniel L E WatersRobert J Henry
Jul 11, 2013·Photosynthesis Research·James O BerryChristopher M Mure
Aug 18, 2000·Biochimie·L A Allison
Dec 26, 2012·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Michael HackenbergPeter Langridge
Apr 27, 2004·Journal of Experimental Botany·Cathrine LilloSatu Oltedal
Aug 23, 2008·Plant & Cell Physiology·Sunok MoonGynheung An
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·P. LeonS. Mackenzie
Jan 25, 2014·Molecular Biology and Evolution·Jeanmaire MolinaMichael D Purugganan
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·K IsonoH Kobayashi
May 30, 2012·The Plant Journal : for Cell and Molecular Biology·Jiang ZhangRalph Bock
May 8, 2015·Molecular Genetics and Genomics : MGG·Chunzhen ChengGuangyan Zhong
May 31, 2015·Plant Science : an International Journal of Experimental Plant Biology·Chao HuangZhong-Nan Yang
Sep 24, 2002·The Journal of Biological Chemistry·Irena SherametiRalf Oelmuller
Mar 20, 1999·Biochemical and Biophysical Research Communications·G K AgrawalM Shirai

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