PMID: 9443377Aug 1, 1997Paper

Evidence for two distinct members of the amylase gene family in the yellow fever mosquito, Aedes aegypti

Insect Biochemistry and Molecular Biology
G L GrossmanA A James

Abstract

Genomic DNA fragments encoding a salivary gland-specific alpha-amylase gene, Amylase I (Amy I), and an additional amylase, Amylase II (AmyII) of the yellow fever mosquito, Aedes aegypti, were isolated and characterized. Two independently isolated DNA fragments, G34-F and G34-14A, encode polymorphic alleles of Amy I. A 3.2 kilobase (kb) EcoR I fragment of G34-F, F2, has been sequenced in its entirety and contains 832 base pairs (bp) of the 5'-end, non-coding and putative promoter regions that are adjacent to 2.4 kb of the Amy I coding region. One intron, 59 bp in length, is found towards the 3'-end of the clone. A third genomic clone, 3A, corresponding to Amy II, was sequenced and shown not to contain the primary DNA sequence that encodes the 260 amino acid region that uniquely characterizes the amino terminal end of the Amy I product. Amy I was assigned by restriction fragment length polymorphism (RFLP) mapping to chromosome 2 (23.0 cM) and Amy II to chromosome 1 (44.0 cM). Amy I and Amy II are highly polymorphic and there may be multiple linked copies at each locus. Comparisons between Amy I and Amy II are presented for the putative promoter and conceptual translation products. The identification of two distinct amylase genes ...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 1, 1992·Genetical Research·J L Da LageJ R David
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·G J PopeT A Bremner
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B J SilverJ H Nilson
Mar 1, 1988·Molecular and Cellular Biology·D L GumucioM H Meisler
Jan 1, 1987·Journal of Molecular Evolution·D F Feng, R F Doolittle
Jan 1, 1987·Journal of Molecular Evolution·D A HickeyG Ben-David
Mar 11, 1988·Nucleic Acids Research·H S Bilofsky, C Burks
Jan 1, 1986·Bulletin of Mathematical Biology·S F Altschul, B W Erickson
Jul 5, 1985·Journal of Molecular Biology·G von Heijne
Jun 14, 1968·Annals of the New York Academy of Sciences·Z Ogita
May 25, 1984·Science·B de CrombruggheH Buc
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·D E ChampagneA A James
Jul 1, 1993·The Journal of Heredity·D W SeversonB M Christensen
Jan 1, 1993·Insect Biochemistry and Molecular Biology·L Cherbas, P Cherbas
Jul 1, 1996·Molecular Biology and Evolution·A PopadićW W Anderson
Oct 1, 1996·Journal of Molecular Evolution·J L Da LageM L Cariou
Jan 1, 1953·Advances in Genetics·H KIKKAWA
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B F Benkel, D A Hickey
Jan 1, 1988·Genetics, Selection, Evolution : GSE·M Laulier

❮ Previous
Next ❯

Citations

Nov 14, 2002·Insect Biochemistry and Molecular Biology·Kendall C KrebsQue Lan
Sep 17, 2009·Insect Molecular Biology·S A BernhardtW C Black
Jun 4, 2011·Archives of Insect Biochemistry and Physiology·Ivana ViktorinovaMichal Zurovec
May 19, 2004·Insect Biochemistry and Molecular Biology·José M C RibeiroJesus G Valenzuela
Mar 22, 2016·PloS One·José M C RibeiroEric Calvo
May 1, 2019·Cell and Tissue Research·Michiel HoltofJozef Vanden Broeck
Jan 17, 2020·Environmental Microbiology·Morgane GuéganClaire Valiente Moro
Oct 12, 2018·International Journal of Insect Science·Jean-Luc Da Lage
Oct 11, 2005·The Journal of Experimental Biology·Bruno ArcàJosé M C Ribeiro
Mar 23, 2000·Insect Biochemistry and Molecular Biology·J M RibeiroR Charlab
Jan 27, 2009·Journal of Proteome Research·John F AndersenJosé M C Ribeiro

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.