PMID: 11897841Mar 19, 2002Paper

Evidence for two distinct processes in the final stages of neurotransmitter release as detected by binomial analysis in calcium and strontium solutions

The Journal of Physiology
Timothy J Searl, E M Silinsky

Abstract

The statistical parameters underlying acetylcholine (ACh) release were studied using Ca(2+) and Sr(2+) ions to promote ACh secretion. Experiments were performed at frog neuromuscular junctions using electrophysiological recording techniques. Increases in asynchronous ACh release, reflected as the frequency of occurrence of miniature end-plate potentials (MEPP(f)), were evoked by high potassium depolarization in either Ca(2+) or Sr(2+) solutions. Increases in MEPP(f) mediated by Ca(2+) were of very low probability and well-described by a Poisson distribution whilst similar MEPP(f) increases mediated by Sr(2+) were best described as a simple binomial distribution. From the binomial distribution in Sr(2+) solutions, values for the average probability of release (p) and the number of releasable ACh quanta (n) may be determined (whereby mean MEPP(f) = np). In Sr(2+) solutions, values of p were independent of both bin width and of the value of n, suggesting that both n and p were stationary. Calculations of p using the simple binomial distribution in Sr(2+) solutions gave theoretical values for the third moment of the mean which were indistinguishable from the experimental distribution. These results, in conjunction with Monte Carlo ...Continue Reading

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Citations

Oct 17, 2002·British Journal of Pharmacology·Eugen BrailoiuNae J Dun
Aug 17, 2010·The Journal of Pharmacology and Experimental Therapeutics·T J Searl, E M Silinsky
Nov 27, 2009·Journal of Neurophysiology·Xueyong WangMark M Rich
Apr 4, 2008·Journal of Neurophysiology·Timothy J Searl, Eugene M Silinsky
Nov 30, 2007·Journal of Neuroscience Methods·Maria Bykhovskaia
Apr 25, 2003·British Journal of Pharmacology·Eugene M Silinsky, Timothy J Searl
Jan 11, 2007·Journal of Neurochemistry·Ellya A BukharaevaLev G Magazanik
Nov 24, 2017·The Indian Journal of Medical Research·Emine Elif AltuntasMelih Akyol

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