PMID: 8457569Mar 30, 1993Paper

Evidence for two pools of cholesterol in the Acholeplasma laidlawii strain B membrane: a deuterium NMR and DSC study

Biochemistry
M A MonckP R Cullis

Abstract

Recent investigations have indicated that there exists a well-defined range of membrane hydrocarbon order compatible with good growth of the microorganism Acholeplasma laidlawii B [Monck, M., Bloom, M., Lafleur, M., Lewis, R. N. A. H., McElhaney, R. N., & Cullis, P. R. (1992) Biochemistry 31, 10037-10043]. Since cholesterol increases hydrocarbon order in membranes, it was of interest to examine the effect of cholesterol on the hydrocarbon order and growth characteristics of A. laidlawii B. Cholesterol is normally absent from A. laidlawii membranes since it is neither biosynthesized nor required for the growth or survival of the microorganism. However, cholesterol will be incorporated into the membrane if exogenously supplied to the A. laidlawii culture. For membranes prepared from cells grown in the presence of cholesterol, chemical determinations indicated cholesterol represented as much as 40 mol% of the total membrane lipid. However, 2H NMR order parameter measurements and DSC studies of the same membrane preparation suggested that cholesterol was present at significantly lower levels (approximately 10-15 mol%) in the membrane lipid bilayer. Further incorporation of cholesterol into the A. laidlawii lipid bilayer was found t...Continue Reading

References

Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M RohmerG Ourisson
Aug 1, 1977·Quarterly Reviews of Biophysics·J Seelig
Aug 1, 1991·Quarterly Reviews of Biophysics·M BloomO G Mouritsen
Oct 1, 1991·Biochimica Et Biophysica Acta·W J JohnsonM C Phillips
Jan 1, 1990·European Biophysics Journal : EBJ·M LafleurM Bloom
Jun 24, 1987·Biochimica Et Biophysica Acta·M C PhillipsG H Rothblat
Sep 1, 1986·Chemistry and Physics of Lipids·E J Dufourc, I C Smith
Nov 1, 1974·Lipids·C Y HsiaoD B Wetlaufer
Dec 21, 1981·Biochimica Et Biophysica Acta·D D Koblin, H H Wang
Jan 27, 1984·Biochimica Et Biophysica Acta·R N McElhaney
Mar 21, 1983·Biochimica Et Biophysica Acta·J H Davis
Jan 1, 1983·CRC Critical Reviews in Biochemistry·K E Bloch
Sep 17, 1984·FEBS Letters·P J DavisS Rottem
Nov 9, 1983·Biochimica Et Biophysica Acta·E C KeluskyI C Smith
May 1, 1982·Reviews of Infectious Diseases·I KahaneJ Feldner
Sep 1, 1960·Clinica Chimica Acta; International Journal of Clinical Chemistry·D WATSON

❮ Previous
Next ❯

Citations

Aug 31, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Zhong-Kai CuiMichel Lafleur
Jan 1, 1995·Molecular Membrane Biology·M Bloom, J L Thewalt
Dec 26, 2001·Biophysical Journal·Michael R BrzustowiczStephen R Wassall
Mar 4, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Thida PhoeungMichel Lafleur
Sep 6, 1994·Chemistry and Physics of Lipids·R Welti, M Glaser
Jul 6, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Zhong-Kai CuiMichel Lafleur
Sep 15, 2017·Chemical Reviews·Trivikram R MoluguMichael F Brown
Aug 25, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Jaclin Ouimet, Michel Lafleur

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.