Evidence for two preferred hairpin folding patterns in d(CGG).d(CCG) repeat tracts in vivo

Journal of Molecular Biology
J M Darlow, D R Leach

Abstract

Unusual DNA secondary structures have been implicated in the expansion of trinucleotide repeat tracts that has been found to be responsible for a growing number of human inherited disorders and folate-sensitive fragile chromosome sites. By inserting trinucleotide repeat sequences into a palindromic clamp in lambda phage we are able to investigate their tendencies to form hairpins in vivo in any particular alignment and with odd or even numbers of repeat units in the hairpin. We previously showed that with d(CAG).d(CTG) repeat tracts there was a markedly greater tendency to form hairpins with even numbers of repeat units than with odd numbers, whereas d(GAC).d(GTC) repeats showed no such alternation despite having the same base composition. We expected that d(CGG).d(CCG) repeats, might show the same pattern as d(CAG).(CTG) repeats since they are also involved in trinucleotide repeat expansion disorders. The pattern was not so clear and we wondered whether this might be because d(CGG).d(CCG) repeats have more than one possible alignment in which they could self-anneal. We now present results for all three alignments, which suggest that while even-membered hairpins are preferred in the frame d(CGG).d(CCG), hairpins with odd number...Continue Reading

References

Dec 1, 1992·Current Opinion in Biotechnology·R R Sinden, R D Wells
Jul 25, 1995·Nucleic Acids Research·A YuM Mitas
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·X ChenG Gupta
Dec 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D R Leach
Oct 25, 1994·Nucleic Acids Research·A Davison, D R Leach
Mar 8, 1996·Science·S T Warren
Feb 9, 1996·The Journal of Biological Chemistry·R D Wells
Feb 6, 1998·Journal of Molecular Biology·J M Darlow, D R Leach

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Citations

Jul 13, 2012·The Journal of Biological Chemistry·Anasheh HalabiEd Grabczyk
May 15, 2001·The EMBO Journal·E VigueraS D Ehrlich
Jan 14, 2004·Nucleic Acids Research·Petr FojtíkMichaela Vorlícková
Dec 4, 2004·Nucleic Acids Research·Vera I HashemRichard R Sinden
Oct 16, 2004·Molecular and Cellular Biology·Bertrand Llorente, Lorraine S Symington
Feb 6, 1998·Journal of Molecular Biology·J M Darlow, D R Leach
Nov 17, 2007·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Arthur E Wood, G Reid Bishop
Jan 22, 2004·FEMS Immunology and Medical Microbiology·Christopher D BaylissE Richard Moxon
May 29, 2001·Journal of Biomedical Optics·L GearheartC J Murphy
Apr 3, 2002·Journal of Biosciences·Richard R SindenLuda S Shlyakhtenko
Feb 24, 2001·Journal of Molecular Biology·E M LeProustC E Pearso
Apr 5, 2018·The Journal of Physical Chemistry. B·Feng PanCeleste Sagui

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