PMID: 7543192Mar 1, 1995Paper

Evidence for two types of non-NMDA receptors in rat cerebellar Purkinje cells maintained in slice cultures

Neuropharmacology
A RenardE Audinat

Abstract

Pharmacological properties of non-NMDA receptors were investigated in Purkinje cells grown in rat cerebellar slice cultures and recorded in the whole-cell configuration of the patch-clamp technique. Dose-response curves for AMPA and domoate suggest that AMPA, in the concentration range tested, activated only AMPA receptors whereas, domoate activated two types of receptors, probably AMPA and kainate receptors, with EC50 values of 8 and 0.5 microM, respectively. The Scatchard analysis of the dose-response relationship for domoate also suggest that both kainate and AMPA receptors were activated by domoate with approximate affinities of 5 and 0.07 microM-1, respectively. The non-competitive non-NMDA receptors antagonist, GYKI 52466, reduced the amplitude of both AMPA- and domoate-activated currents, with a greater potency in reducing currents evoked by AMPA (IC50 = 10 microM) than those induced by domoate (IC50 = 105 microM). These results suggest that, in addition to AMPA receptors, Purkinje cells express kainate receptors and that these two types of non-NMDA receptors can be distinguished from each other on the basis of several pharmacological properties, including affinity for AMPA, domoate and GYKI 52466.

References

Jul 28, 1992·FEBS Letters·H LomeliP H Seeburg
Aug 1, 1992·Neuron·B LambolezJ Rossier
Apr 15, 1992·The Journal of Comparative Neurology·R S Petralia, R J Wenthold
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D E Pellegrini-GiampietroR S Zukin
Aug 3, 1990·Science·K KeinänenP H Seeburg
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L O TrussellG D Fischbach
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L O TrussellG D Fischbach
Jan 1, 1982·Journal of Neurochemistry·T HonoréP Krogsgaard-Larsen
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J LermaB Mellström
Aug 15, 1993·European Journal of Pharmacology·T H JohansenE O Nielsen

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Citations

Jan 1, 1997·Trends in Neurosciences·J LermaO Herreras
Sep 15, 1998·Trends in Neurosciences·H DanielF Crépel
Sep 23, 2000·Brain Research. Molecular Brain Research·D J FogartyC Matute
Sep 27, 2003·Progress in Neurobiology·James E Huettner
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·I BureauC Mulle
Oct 9, 2015·Journal of Neurochemistry·Izumi Watanabe-IidaKenji Sakimura
Jul 10, 1997·Nature·M Vignes, G L Collingridge
Jan 1, 1997·Journal of Neurophysiology·P D LukasiewiczJ E Lawrence
Jun 7, 2011·Neuropharmacology·Pervin K IseriNurbay Ates
Oct 6, 1998·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·C LevenesF Crépel

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