PMID: 8583279Dec 1, 1995Paper

Evidence for UV-B-induced DNA degradation in Euglena gracilis mediated by activation of metal-dependent nucleases

Journal of Photochemistry and Photobiology. B, Biology
R ScheuerleinD P Häder

Abstract

It is demonstrated that in vivo irradiation with artificial UV-B for several hours significantly reduces the amount of large DNA extractable from immobilized Euglena in comparison with non-irradiated controls. This UV-B effect can be eliminated by a drastic reduction of the divalent ion concentration in the extracellular medium, i.e. the substitution of the culture medium by Tris-buffered agarose. Moreover, in vitro degradation of large DNA is demonstrated for crude protein extracts isolated from non-irradiated or UV-B-irradiated Euglena. The nuclease activity is shown for both crude protein extracts and purified nucleases; in both cases, two protein bands possessing nuclease activity are obtained with apparent molecular masses of 26 and 40 kDa and their activity is inhibited by specific nuclease inhibitors, i.e. aurintricarboxylic acid and ATP, applied at a concentration as low as 10(-8) M. Moreover, in vitro, nuclease activity clearly depends on the pH, with an optimum around pH 4.5, and on the ion composition of the extracellular medium. A strong stimulating effect is shown for Ca2+ with an optimum around 10(-4) M; this effect is potentiated by Zn2+ and Mn2+, but strongly counteracted by Mg2+ and the calmodulin inhibitors tr...Continue Reading

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Citations

Nov 25, 2000·Journal of Experimental Marine Biology and Ecology·S J NewmanD Ritz
May 29, 2002·Journal of Photochemistry and Photobiology. B, Biology·Helen PalmerTetsuya Suzuki
Apr 18, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Shogo Ikeda, Norifumi Takata
Jun 6, 2003·Mutation Research·Simone HuettenbrennerGeorg Krupitza
Jul 25, 2007·Photochemistry and Photobiology·Peter RichterDonat-P Häder
Mar 4, 2015·Antimicrobial Agents and Chemotherapy·Carmen M Martín-NavarroJacob Lorenzo-Morales
Nov 3, 2007·Microbiology·J D'Artagnan VillalbaD Guillermo Pérez Ishiwara
Dec 25, 2019·Parasites & Vectors·Atteneri López-ArencibiaJosé E Piñero
Jul 13, 2004·Trends in Parasitology·Paul A NguewaJosé M Pérez

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