PMID: 8972699Dec 4, 1996Paper

Evidence from incorporation experiments for an anionic channel of small conductance at the apical membrane of the rabbit distal tubule

Biochimica Et Biophysica Acta
N DenicourtR Sauvé

Abstract

Many of the hormone-regulated ion transport processes in distal nephron involve transcellular pathways which require a passive entry of ions at the apical membrane of the distal tubule cells. To investigate molecular mechanisms underlying the ionic permeability of the distal tubule apical membrane, a study was undertaken in which vesicles prepared from apical membranes from isolated rabbit distal tubules were fused onto a planar lipid bilayer. These experiments led to the identification of several ionic channels including a Cl(-)-permeable channel of 14 pS with a Na+ over Cl- permeability ratio, PNa/PCl < 0.09. The open channel probability (Po) showed a weak voltage dependency with Po increasing slightly at negative potential values (intracellular (trans) relative to extracellular (cis) for right-side-out vesicles). Channel activity was inhibited by NPPB at high concentrations (> 100 microM) and by DIDS (300 microM). A small inhibitory effect was also observed in the presence of DPC at concentrations ranging from 200 microM to 500 microM. The presence of SO4(2-) (32 mmol/l) in the trans solution caused a complete inhibition of channel activity, but no modification of channel behaviour was observed with the non-selective channel...Continue Reading

Citations

Apr 2, 2003·The Journal of General Physiology·Stéphane LourdelJacques Teulon
Jun 27, 2002·The Journal of General Physiology·Manuel SimoesRémy Sauvé
Aug 24, 1999·The Journal of Physiology·U BanderaliJ Ehrenfeld
Dec 19, 2002·Biochimica Et Biophysica Acta·Olivier PeyronnetJean-Louis Schwartz

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