Evidence of α-helical coiled coils and β-sheets in hornet silk

Journal of Structural Biology
Tsunenori KamedaAndreas K Schaper

Abstract

α-Helical coiled coil and β-sheet complexes are essential structural building elements of silk proteins produced by different species of the Hymenoptera. Beside X-ray scattering at wide and small angles we applied cryo-electron diffraction and microscopy to demonstrate the presence and the details of such structures in silk of the giant hornet Vespa mandarinia japonica. Our studies on the assembly of the fibrous silk proteins and their internal organization in relation to the primary chain structure suggest a 172 Å pitch supercoil consisting of four intertwined alanine-rich α-helical strands. The axial periodicity may adopt even multiples of the pitch value. Coiled coil motifs form the largest portion of the hornet silk structure and are aligned nearly parallel to the cocoon fiber axis in the same way as the membrane-like parts of the cocoon are molecularly orientated in the spinning direction. Supercoils were found to be associated with β-crystals, predominantly localized in the l-serine-rich chain sequences terminating each of the four predominant silk proteins. Such β-sheet blocks are considered resulting from transformation of random coil molecular sequences due to the action of elongational forces during the spinning process.

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Citations

Oct 31, 2015·Journal of Structural Biology·R D Bruce Fraser, David A D Parry
Jun 5, 2015·Proceedings. Biological Sciences·Andrew A WalkerTara D Sutherland
Feb 26, 2015·Insect Biochemistry and Molecular Biology·Jakkrawut MaitipTara D Sutherland
Feb 3, 2018·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Martin HumenikThomas Scheibel
Nov 1, 2017·Biomacromolecules·Taiyo YoshiokaAndreas K Schaper

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