Evidence of a growth hormone-releasing hormone-like molecule in salmon brain, Oncorhynchus keta and O. kisutch

General and Comparative Endocrinology
D B Parker, N M Sherwood

Abstract

An antiserum with conformational specificity to human growth hormone-releasing hormone (hGH-RH 1-44 NH2) was produced and used to develop a radioimmunoassay to detect immunoreactive (ir) GH-RH in brain extracts of salmon, guinea pig, and mouse. Evidence of an immunoreactive GH-RH from salmon brain extracts with a retention time on reverse-phase high-performance liquid chromatography (HPLC) distinct from hGH-RH 1-44 is presented. Salmon irGH-RH from crude extracts elutes 1-2 min earlier than hGH-RH 1-44 (NH2) in a gradient HPLC system, whereas affinity-purified salmon irGH-RH elutes 12-13 min earlier in a near-isocratic system, suggesting that the salmon molecule is more hydrophilic.

Citations

Oct 24, 2002·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Leandro Andrés MirandaGustavo Manuel Somoza

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