PMID: 9525648Apr 3, 1998Paper

Evidence of a role for phosphatidylinositol 3-kinase activation in the blocking of apoptosis by polyomavirus middle T antigen

Journal of Virology
J DahlT L Benjamin

Abstract

A polyomavirus mutant (315YF) blocked in binding phosphatidylinositol 3-kinase (PI 3-kinase) has previously been shown to be partially deficient in transformation and to induce fewer tumors and with a significant delay compared to wild-type virus. The role of polyomavirus middle T antigen-activated PI 3-kinase in apoptosis was investigated as a possible cause of this behavior. When grown in medium containing 1D-3-deoxy-3-fluoro-myo-inositol to block formation of 3'-phosphorylated phosphatidylinositols, F111 rat fibroblasts transformed by wild-type polyomavirus (PyF), but not normal F111 cells, showed a marked loss of viability with evidence of apoptosis. Similarly, treatment with wortmannin, an inhibitor of PI 3-kinase, stimulated apoptosis in PyF cells but not in normal cells. Activation of Akt, a serine/threonine kinase whose activity has been correlated with regulation of apoptosis, was roughly twofold higher in F111 cells transformed by either wild-type virus or mutant 250YS blocked in binding Shc compared to cells transformed by mutant 315YF. In the same cells, levels of apoptosis were inversely correlated with Akt activity. Apoptosis induced by serum withdrawal in Rat-1 cells expressing a temperature-sensitive p53 was sho...Continue Reading

References

Apr 3, 1992·Cell·G I EvanD C Hancock
Jan 1, 1991·Cancer Chemotherapy and Pharmacology·G PowisA P Kozikowski
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P F JonesB A Hemmings
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G CarmichaelT L Benjamin
Feb 1, 1995·Current Opinion in Genetics & Development·Y Shen, T E Shenk
May 26, 1995·The Journal of Biological Chemistry·W SuT M Roberts
Jan 1, 1995·Advances in Virus Research·E S Razvi, R M Welsh
Feb 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M K CollinsA López-Rivas
Nov 19, 1993·Cell·W S el-DeiryB Vogelstein
Apr 1, 1993·Genes & Development·M Debbas, E White
Jan 1, 1993·Annual Review of Biochemistry·A J Levine
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·A T YoungT L Benjamin
Nov 1, 1995·Molecular Biology of the Cell·Y IshizakiM C Raff

❮ Previous
Next ❯

Citations

Aug 24, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M L OliveiraM C Sogayar
Nov 5, 1999·Annual Review of Microbiology·A RoulstonP E Branton
Apr 21, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Silvina SímulaNorberto Sanjuan
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·K A Gottlieb, L P Villarreal
Aug 21, 2002·Journal of Virology·Dilip DeyThomas L Benjamin
Sep 27, 2001·Viral Immunology·J M Moser, A E Lukacher
Sep 29, 2005·Journal of Virology·Jean DahlThomas L Benjamin
Nov 18, 2016·Journal of Virology·Deborah DenisBrian S Schaffhausen
May 28, 2016·Viruses·Valerie Le SageAndrew J Mouland
Aug 3, 2012·The Journal of General Virology·Andrew S Turnell, Roger J Grand
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M AokiP K Vogt
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Michele M Fluck, Brian S Schaffhausen
Feb 21, 2006·FEMS Immunology and Medical Microbiology·Tetsuya MizutaniShigeru Morikawa
Jan 9, 1999·Journal of Virology·J P CarrollT L Benjamin
Jan 9, 2001·The Journal of Investigative Dermatology. Symposium Proceedings·R Klafter, J L Arbiser
Jul 18, 2001·Journal of the American Chemical Society·M Hoffmann, J Rychlewski

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

BK Virus Infection

BK virus infection is a significant complication of modern immunosuppression used in kidney transplantation. Discover the latest research on BK virus infection here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis