Evidence of altered histone interactions, as investigated by removal of histones, in chromatin isolated from rat liver nuclei by a conventional method.

Nucleic Acids Research
H FenskeR Lindigkeit

Abstract

It is shown that the release of the slightly lysine-rich histones f2a2 and f2b by 0.4 M ammonium sulfate from conventionally isolated chromatin is diminished in comparison to the lysed nuclei. The change in extractability is further demonstrated by the application of ethidium bromide. At a molar input ratio of 0.09 (moles ethidium bromide/moles nucleotide) and 0.4 M ammonium sulfate the slightly lysine-rich histones are released from the chromatin to 70 - 80% if the lysed nuclei are used. At 0.1 M ammonium sulfate ethidium bromide effected also a release of 50 % of histone f1. Comparable effects could not be observed with chromatin prepared in a conventional way but instead a tendency towards loss of histone f3 in the presence of ethidium bromide was observed.

References

Jan 25, 1974·Science·A L Olins, D E Olins
Aug 15, 1974·Biochimica Et Biophysica Acta·A W ReesE A Lewis
Jan 12, 1973·Nature·E M BradburyH W Rattle
Aug 24, 1973·Biochimica Et Biophysica Acta·K E Reinert
May 24, 1974·Science·R D Kornberg, J O Thomas
Nov 11, 1971·European Journal of Biochemistry·P H ButterworthC J Chesterton
Mar 1, 1969·Archives of Biochemistry and Biophysics·S Panyim, R Chalkley
Mar 28, 1975·Science·M NollR D Kornberg

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Citations

Apr 16, 1982·Molecular Biology Reports·M BöttgerR Lindigkeit
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C T McMurray, K E van Holde
Feb 1, 1976·Nucleic Acids Research·M BöttgerH Fenske
Jul 25, 1979·Nucleic Acids Research·M ErardM Daune
Mar 22, 2014·FEBS Open Bio·Amrita BanerjeeDipak Dasgupta
Jul 1, 1994·Molecular and Cellular Biology·X J Chen, G D Clark-Walker

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