Evidence of an association between human papillomavirus and impaired chemotherapy-induced apoptosis in cervical cancer cells

Gynecologic Oncology
Luis A PadillaLinda F Carson

Abstract

The aim of this study was to determine cervical cancer cell sensitivity to chemotherapy-induced apoptosis based on human papillomavirus (HPV) status. CaSki (HPV-positive) and C33A (HPV-negative) cells were treated with camptothecin or cisplatin. Cellular viability was determined by trypan blue exclusion. Apoptotic indexes were determined by flow cytometric analysis of annexin V labeling and morphological changes. Mitochondrial release of cytochrome c was determined by immunofluorescence using confocal microscopy. Caspase 3 activation and bax expression were assessed by immunoblotting. CaSki cells displayed chemoresistance to both camptothecin and cisplatin. Low response to apoptogenic stimuli was evidenced by a marginal increase in the apoptotic cell fraction after camptothecin treatment (22.9 +/- 2.56%) compared with control (17.8 +/- 1.95%). Cisplatin (14.8 +/- 1.01%) caused a slight decrease in apoptosis. Mitochondrial release of cytochrome c and cleavage of caspase 3 could not be demonstrated in CaSki cells after treatment. Despite p53 mutation, C33A cells were sensitive to the antiproliferative effects of camptothecin and cisplatin. Mean apoptotic events were 17.5 +/- 0.33 for control, 42 +/- 1.76 for cisplatin, and 38.1 +...Continue Reading

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Citations

Jan 5, 2007·Apoptosis : an International Journal on Programmed Cell Death·Pongali B RaghavendraSunil K Manna
May 10, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·X-Y NiuP Wang
Jan 10, 2012·Biochemical Pharmacology·Michelle Martinez-Rivera, Zahid H Siddik
Oct 16, 2007·Gynecologic Oncology·Elena Aréchaga-OcampoNicolás Villegas-Sepúlveda
Oct 13, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Vandana KeskarRichard A Gemeinhart
Aug 15, 2014·Biochemical and Biophysical Research Communications·Hongtao WangJie Zheng
Jul 28, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Kanchan VishnoiAlok C Bharti
Jul 19, 2012·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Yang SunHong Shi
May 29, 2019·The Journal of Obstetrics and Gynaecology Research·Kohei SeoMasaaki Nagatsuka
Jan 17, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Evgenia GubanovaNatalia Issaeva
Jun 14, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Javid Sadri NahandHamed Mirzaei

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