Evidence of an Epigenetic Modification in Cell-cycle Arrest Caused by the Use of Ultra-highly-diluted Gonolobus Condurango Extract

Journal of Pharmacopuncture
Kausik BishayeeAnisur R Khuda-Bukhsh

Abstract

Whether the ultra-highly-diluted remedies used in homeopathy can effectively bring about modulations of gene expressions through acetylation/deacetylation of histones has not been explored. Therefore, in this study, we pointedly checked if the homeopathically-diluted anti-cancer remedy Condurango 30C (ethanolic extract of Gonolobus condurango diluted 10(-60) times) was capable of arresting the cell cycles in cervical cancer cells HeLa by triggering an epigenetic modification through modulation of the activity of the key enzyme histone deacetylase 2 vis-a-vis the succussed alcohol (placebo) control. We checked the activity of different signal proteins (like p21(WAF), p53, Akt, STAT3) related to deacetylation, cell growth and differentiation by western blotting and analyzed cell-cycle arrest, if any, by fluorescence activated cell sorting. After viability assays had been performed with Condurango 30C and with a placebo, the activities of histone de-acetylase (HDAC) enzymes 1 and 2 were measured colorimetrically. While Condurango 30C induced cytotoxicity in HeLa cells in vitro and reduced HDAC2 activity quite strikingly, it apparently did not alter the HDAC1 enzyme; the placebo had no or negligible cytotoxicity against HeLa cells ...Continue Reading

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Citations

Aug 29, 2017·Homeopathy : the Journal of the Faculty of Homeopathy·Jenifer Pendiuk GonçalvesCarolina Camargo de Oliveira
Jul 31, 2016·Homeopathy : the Journal of the Faculty of Homeopathy·José Roberto Pereira GuedesVera L Capelozzi
Dec 20, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Ilaria MarottiGiovanni Dinelli
Dec 12, 2017·Dose-response : a Publication of International Hormesis Society·Andrea Dei
Jan 21, 2016·Dose-response : a Publication of International Hormesis Society·Elisabetta BigagliPiero Dolara
Mar 3, 2020·Homeopathy : the Journal of the Faculty of Homeopathy·Andrea Dei
Dec 22, 2020·Homeopathy : the Journal of the Faculty of Homeopathy·Andreza Pereira Dos SantosLeoni Villano Bonamin
Nov 18, 2021·Homeopathy : the Journal of the Faculty of Homeopathy·Ayesha LoonatRahul Chandran

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Methods Mentioned

BETA
histone acetylation
ELISA
flow-cytometry

Software Mentioned

image J
SPSS

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