Evidence of decreased gap junction coupling between astrocytes and oligodendrocytes in the anterior cingulate cortex of depressed suicides

Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology
A TantiNaguib Mechawar

Abstract

Glial dysfunction is a major pathophysiological feature of mood disorders. While altered astrocyte (AS) and oligodendrocyte-lineage (OL) functions have been associated with depression, the crosstalk between these glial cell types has never been assessed in that context. AS are potent regulators of myelination, in part through gap junction (GJ) channels formed by the heterotypic coupling of AS-specific (Cx30 and Cx43) and OL-specific (Cx32 and Cx47) connexins. This study therefore aimed at addressing the integrity of AS/OL coupling in the anterior cingulate cortex (ACC) of depressed suicides. Using immunofluorescence and confocal imaging, we characterized the distribution of Cx30 and mapped its expression onto OL somas, myelinated axons, and brain vasculature in postmortem brain samples from depressed suicides (N = 48) and matched controls (N = 23). Differential gene expression of key components of the GJ nexus was also screened through RNA-sequencing previously generated by our group, and validated by quantitative real-time PCR. We show that Cx30 expression localized onto OL cells and myelinated fibers is decreased in deep cortical layers of the ACC in male-depressed suicides. This effect was associated with decreased expressio...Continue Reading

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Citations

Jan 8, 2021·Neurochemical Research·Xiaolu ZhangSinead M O'Donovan
Feb 23, 2021·Frontiers in Psychiatry·Liam Anuj O'LearyNaguib Mechawar

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Methods Mentioned

BETA
confocal microscopy
PCR
RNA-seq
co-immunoprecipitation

Software Mentioned

QuantStudio Real - Time PCR
SPSS
ImageJ
DESeq2
BLAST
StatSoft
Prism
Statistica
GraphPad
Primer

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